Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23797-104] to PVRIG/CD112R
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Product nameAnti-PVRIG/CD112R antibody [EPR23797-104]
See all PVRIG/CD112R primary antibodies
DescriptionRabbit monoclonal [EPR23797-104] to PVRIG/CD112R
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC,IHC-P,IP or WB
Species reactivityReacts with: Human
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
- Flow cyt: HEK-293T and human PBMC cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Information by UniProt
- Poliovirus receptor-related immunoglobulin domain-containing protein antibody
- PVRIG antibody
- PVRIG_HUMAN antibody
- Transmembrane protein PVRIG antibody
Flow cytometric analysis of 2% paraformaldehyde fixed, 0.1% Tween-20 permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-tagged PVRIG/CD112R construct cells labelling PVRIG/CD112R with ab256808 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with rabbit IgG and ab256808, then fixed with 2% PFA followed by intracellularly staining with anti-myc tag conjugated to AlexaFluor®647.
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling PVRIG/CD112R with ab256808 at 1/500 dilution (0.1ug)(Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab256808. Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab256808 has not yet been referenced specifically in any publications.