Product nameAnti-PVRL1/NECTIN1 antibody
See all PVRL1/NECTIN1 primary antibodies
DescriptionRabbit polyclonal to PVRL1/NECTIN1
Tested applicationsSuitable for: IHC-FoFr, WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Pig, Xenopus laevis
- This antibody gave a positive signal in the following lysates: Brain (Mouse) Tissue; Testis (Human) Tissue; HeLa Whole Cell; SK N SH Whole Cell; Hela Whole Cell - Staurosporine Treated (24hr, 500nM)
This product was previously labelled as PVRL1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab66985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 57 kDa). Abcam recommends using milk as the blocking agent.|
|IHC-P||Use at an assay dependent concentration. PubMed: 24130898|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21769484|
FunctionPromotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4.
Involvement in diseaseDefects in PVRL1 are the cause of ectodermal dysplasia Margarita Island type (EDMI) [MIM:225060]; also known as Zlotogora-Ogur syndrome, cleft lip/palate-ectodermal dysplasia syndrome (CLPED1) or ectodermal dysplasia 4. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDMI is an autosomal recessive syndrome characterized by the association of cleft lip/palate, ectodermal dysplasia (sparse short and dry scalp hair, sparse eyebrows and eyelashes), and partial syndactyly of the fingers and/or toes. Two thirds of the patients do not manifest oral cleft but present with abnormal teeth and nails.
Defects in PVRL1 are the cause of non-syndromic orofacial cleft type 7 (OFC7) [MIM:225060]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
Sequence similaritiesBelongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD111 antibody
- CD111 antigen antibody
- CLPED1 antibody
All lanes : Anti-PVRL1/NECTIN1 antibody (ab66985) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Mouse Dorsal Root Ganglion
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 210 kDa, 35 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
PVRL1/NECTIN1 protein is known to be glycosylated and it migrates at a higher molecular weight than the predicted band size of 57kDa (Swiss-prot data), as demonstrated by PMID 15731277. Abcam recommends using 3% milk as the blocking agent with ab66985
IHC-FoFr image of PVRL1/NECTIN1 (ab66985) staining on Rat hippocampus and Striatum sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
This product has been referenced in:
- Waters AM et al. Effect of Repeat Dosing of Engineered Oncolytic Herpes Simplex Virus on Preclinical Models of Rhabdomyosarcoma. Transl Oncol 9:419-430 (2016). IHC ; Human . Read more (PubMed: 27751346) »
- Cheshenko N et al. Herpes simplex virus type 2 glycoprotein H interacts with integrin avß3 to facilitate viral entry and calcium signaling in human genital tract epithelial cells. J Virol 88:10026-38 (2014). Read more (PubMed: 24942591) »