Overview

  • Product name
    Anti-PVRL4 [EPR15613-68] antibody
    See all PVRL4 primary antibodies
  • Description
    Rabbit monoclonal [EPR15613-68] to PVRL4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human PVRL4 aa 50-200. The exact sequence is proprietary.
    Database link: Q96NY8

  • Positive control
    • T47D, MCF7 and Human placenta lysates; Human placenta tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15613-68
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 44, 55, 66 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells.
  • Tissue specificity
    Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
  • Involvement in disease
    Defects in PVRL4 are the cause of ectodermal dysplasia-syndactyly syndrome type 1 (EDSS1) [MIM:613573]. EDSS1 is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSS1 is characterized by the association of hair and teeth abnormalities with cutaneous syndactyly of the hands and/or feet. Hair morphologic abnormalities include twists at irregular intervals (pilli torti) and swelling along the shafts, particularly associated with areas of breakage. Dental findings consist of abnormally widely spaced teeth, with peg-shaped and conical crowns. Patients have normal sweating.
  • Sequence similarities
    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE.
  • Cellular localization
    Cell membrane. Cell junction > adherens junction. Colocalizes with MLLT4 at cadherin-based adherens junctions and Secreted. The secreted form is found in breast tumor patients.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686K05193 antibody
    • EDSS1 antibody
    • Ig superfamily receptor LNIR antibody
    • Nectin 4 antibody
    • Nectin-4 antibody
    • poliovirus receptor-related 4 antibody
    • Processed poliovirus receptor-related protein 4 antibody
    • PRR4 antibody
    • pvrl4 antibody
    • PVRL4_HUMAN antibody
    see all

Images

  • All lanes : Anti-PVRL4 [EPR15613-68] antibody (ab192033) at 1/1000 dilution

    Lane 1 : T47D lysate
    Lane 2 : MCF7 lysate
    Lane 3 : Human placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

  • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PVRL4 with ab192033 at 1/3000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    Inset: Negative control: using PBS instead of primary antibody.

References

This product has been referenced in:
  • Zhang Y  et al. High expression of Nectin-4 is associated with unfavorable prognosis in gastric cancer. Oncol Lett 15:8789-8795 (2018). Read more (PubMed: 29805618) »
  • Nishiwada S  et al. Nectin-4 expression contributes to tumor proliferation, angiogenesis and patient prognosis in human pancreatic cancer. J Exp Clin Cancer Res 34:30 (2015). WB . Read more (PubMed: 25888293) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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