Product nameAnti-PWP1 antibody [EPR16130]
See all PWP1 primary antibodies
DescriptionRabbit monoclonal [EPR16130] to PWP1
Tested applicationsSuitable for: IHC-P, Flow Cyt, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PWP1 aa 50-150. The exact sequence is proprietary.
Database link: Q13610
- Jurkat, HeLa, 293, HepG2 cell lysates, Human pancreas tissue, Jurkat cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab190794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 56 kDa).|
FunctionMay play an important role in cell growth and/or transcription.
Tissue specificityHigh levels seen in the placenta, skeletal muscle, kidney and pancreas while lower levels were seen in the heart, brain and lung.
Sequence similaritiesBelongs to the WD repeat PWP1 family.
Contains 5 WD repeats.
- Information by UniProt
- Keratinocyte protein IEF SSP 9502 antibody
- Periodic tryptophan protein 1 homolog antibody
- PWP1 antibody
Anti-PWP1 antibody [EPR16130] (ab190794) at 1/10000 dilution + Jurkat cell lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Additional bands at: 79 kDa. We are unsure as to the identity of these extra bands.
All lanes : Anti-PWP1 antibody [EPR16130] (ab190794) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling PWP1 with ab190794 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow cytometry analysis of PWP1 expression in Jurkat cells using ab190794 at 1/30 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.
ab190794 has been referenced in 2 publications.