Key features and details
- Rabbit polyclonal to PXK
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PXK antibody
See all PXK primary antibodies
DescriptionRabbit polyclonal to PXK
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human PXK aa 528-578. The exact sequence is proprietary.
Database link: Q7Z7A4
- IP: HEK-293T whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab241192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
8 - 15 µl/mg lysate
FunctionBinds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity.
Tissue specificityWidely expressed in all tissues examined except in heart. Isoform 1 is expressed in high levels in the brain, skeletal muscle, spleen and testis. Isoform 7 expression has yet to be demonstrated.
Sequence similaritiesBelongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
Contains 1 PX (phox homology) domain.
Contains 1 WH2 domain.
DomainThe protein kinase domain is predicted to be catalytically inactive.
Cellular localizationCytoplasm. Cell membrane. Also associates with the plasma membrane. Isoform 3 is present throughout the cell.
- Information by UniProt
- FLJ20335 antibody
- K-ATPase antibody
- Modulator of Na antibody
PXK was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, prepared using NETN lysis buffer) using ab241192 at 8 µl per reaction. Western blot was performed on the immunioprecipitate using ab241192 at 1/400 dilution.
Lane 1: ab241192 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241192 has not yet been referenced specifically in any publications.