Overview

  • Product name

  • Description

    Mouse polyclonal to PXR
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-434 of Human PXR (NP_003880.3).

  • Positive control

    • Rat liver tissue lysate; PXR transfected 293T cell line lysate; HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab118336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
  • Tissue specificity

    Expressed in liver, colon and small intestine.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BXR antibody
    • Nr1i2 antibody
    • NR1I2_HUMAN antibody
    • Nuclear receptor subfamily 1 group I member 2 antibody
    • ONR 1 antibody
    • ONR1 antibody
    • Orphan nuclear receptor PAR 1 antibody
    • Orphan nuclear receptor PAR1 antibody
    • Orphan nuclear receptor PXR antibody
    • OTTHUMP00000215173 antibody
    • OTTHUMP00000215174 antibody
    • OTTHUMP00000215175 antibody
    • PAR 1 antibody
    • PAR 2 antibody
    • PAR antibody
    • PAR q antibody
    • PAR1 antibody
    • PAR2 antibody
    • PARq antibody
    • pregnane X nuclear receptor variant 2 antibody
    • Pregnane X receptor antibody
    • PRR antibody
    • PXR antibody
    • SAR antibody
    • Steroid and xenobiotic receptor antibody
    • SXR antibody
    see all

Images

  • Anti-PXR antibody (ab118336) at 1 µg/ml + Rat liver tissue lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 50 kDa

  • All lanes : Anti-PXR antibody (ab118336) at 1 µg/ml

    Lane 1 : PXR transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 50 kDa

  • ab118336 at 10 µg/ml dilution staining PXR in HepG2 cells by Immunofluorescence.

References

This product has been referenced in:

  • Jang M  et al. Study of melatonin-mediated effects on various hepatic inflammatory responses stimulated by IL-6 in a new HepG2-on-a-chip platform. Biomed Microdevices 20:54 (2018). Read more (PubMed: 29946898) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer


I am happy to confirm you that this antibody will work using formaldehyde fixed cells. I can confirm that the image provided on the datasheet was obtained using 4% paraformaldehyde solution as fixative.

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Answer

Thank you for contacting us.

Your credit note ID is *.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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Answer

Thank you for your reply.

I am sorry that the antibody, ab118336 did not perform as stated on the datasheet and I would be happy to process a refund for the cost of the antibody. To be able to complete the refund, would you please be able to provide me with either the Abcam order number, or PO number that was used to purchase the antibody.

As soon as I have that information, I will go ahead and complete the refund.

I look forward to your reply.

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Answer

Thank you for contacting Abcam.

I have attached the protocol that was used to test ab118336 in IF, the protocol is similar to yours but there are a few differences that would be worth trying out.

The antibody is covered under our Abpromise for six months and is guaranteed to work inIF on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

Please let me know if there is anything else I can help you with.

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