Product nameAnti-PXR antibody
See all PXR primary antibodies
DescriptionGoat polyclonal to PXR
SpecificityThis antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3).
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Pig
- HepG2 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
Our Abpromise guarantee covers the use of ab81970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 50 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionNuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
Tissue specificityExpressed in liver, colon and small intestine.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- BXR antibody
- Nr1i2 antibody
- NR1I2_HUMAN antibody
Anti-PXR antibody (ab81970) at 1 µg/ml + HepG2 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 hour
An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide.
ab81970 has not yet been referenced specifically in any publications.