Anti-PYCR1 antibody (ab102601)
Key features and details
- Rabbit polyclonal to PYCR1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PYCR1 antibody
See all PYCR1 primary antibodies -
Description
Rabbit polyclonal to PYCR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 287 of Human PYCR1 (AAH01504).
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Positive control
- WB: Raji cell lysate; IHC-P: Human breast cancer tissue, IF: HeLa whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab102601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
Target
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Relevance
PYCR1 is an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 5831 Human
- Omim: 179035 Human
- SwissProt: P32322 Human
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Alternative names
- P5C antibody
- P5C reductase 1 antibody
- P5C reductase antibody
see all
Images
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Anti-PYCR1 antibody (ab102601) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 33 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling PYCR1 with ab102601 at 1/250 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Immunofluorescence analysis of 2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labeling PYCR1 protein at mitochondria (green) with ab102601 at 1/500 dilution. Blue: Hoechst 33342 staining.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab102601 has been referenced in 1 publication.
- Lu J et al. MiR-328-3p inhibits lung adenocarcinoma-genesis by downregulation PYCR1. Biochem Biophys Res Commun 550:99-106 (2021). PubMed: 33706104