Western blot

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Abreviews
Application
Western blot
Loading amount
36 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (HeLa, RPE-1)
Specification
HeLa, RPE-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 2°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS in 4% milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: LICOR, anti rabbit 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/20000

Detection

Detection method
Near Infrared Fluorescence
Bands
Specific: 34 kDa
Exposure
7 minute(s) and 0 second(s)

Additional data

Additional Notes
lysis buffer: PBS + 0.5% Triton X-100 lane 1: 18 ug Hela extract lane 2: 36 ug Hela extract lane 3: 18 ug RPE-1 extract lane 4: 36 ug RPE-1 extract

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Submitted May 01 2013

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