Application
Western blot
Loading amount
36 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (HeLa, RPE-1)
Specification
HeLa, RPE-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 2°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS in 4% milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: LICOR, anti rabbit 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/20000
Detection
Detection method
Near Infrared Fluorescence
Bands
Specific: 34 kDa
Exposure
7 minute(s) and 0 second(s)
Additional data
Additional Notes
lysis buffer: PBS + 0.5% Triton X-100
lane 1: 18 ug Hela extract
lane 2: 36 ug Hela extract
lane 3: 18 ug RPE-1 extract
lane 4: 36 ug RPE-1 extract
Abcam user community
Verified customer
Submitted May 01 2013