Key features and details
- Rabbit polyclonal to PYCR1 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PYCR1 antibody - C-terminal
See all PYCR1 primary antibodies
DescriptionRabbit polyclonal to PYCR1 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human PYCR1 aa 269-319 (C terminal). The exact sequence is proprietary.
Database link: P32322
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226340 was affinity purified using an epitope specific to PYCR1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 33 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevancePYCR1 is an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion.
- P5C antibody
- P5C reductase 1 antibody
- P5C reductase antibody
All lanes : Anti-PYCR1 antibody - C-terminal (ab226340) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 30 seconds
Cell lysates were prepared using NETN lysis buffer.
PYCR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226340 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226340 at 1 µg/ml.
Lane 1: ab226340 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226340 has not yet been referenced specifically in any publications.