Overview

  • Product name

    Anti-PYCR1 antibody - C-terminal
    See all PYCR1 primary antibodies
  • Description

    Rabbit polyclonal to PYCR1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human PYCR1 aa 269-319 (C terminal). The exact sequence is proprietary.
    Database link: P32322

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226340 was affinity purified using an epitope specific to PYCR1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 33 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    PYCR1 is an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion.
  • Cellular localization

    Mitochondrial
  • Database links

  • Alternative names

    • P5C antibody
    • P5C reductase 1 antibody
    • P5C reductase antibody
    • P5CR 1 antibody
    • P5CR antibody
    • PIG45 antibody
    • PP222 antibody
    • Proliferation inducing protein 45 antibody
    • PYCR 1 antibody
    • PYCR antibody
    • Pyrroline 5 carboxylate reductase 1 antibody
    • Pyrroline 5 carboxylate reductase 1 mitochondrial antibody
    see all

Images

  • All lanes : Anti-PYCR1 antibody - C-terminal (ab226340) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 33 kDa


    Exposure time: 30 seconds


    Cell lysates were prepared using NETN lysis buffer.

  • PYCR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226340 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226340 at 1 µg/ml.

    Lane 1: ab226340 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab226340 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226340.
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