Overview

  • Product name
    Anti-PYCR2 antibody
    See all PYCR2 primary antibodies
  • Description
    Rabbit polyclonal to PYCR2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-320 of Human PYCR2 (NP_037460.2).

  • Positive control
    • HeLa cell lysate; PYCR2 transfected 293T cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab103535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.

Target

  • Function
    Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH.
  • Tissue specificity
    Detected in erythrocytes (at protein level).
  • Pathway
    Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
  • Sequence similarities
    Belongs to the pyrroline-5-carboxylate reductase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • 1810018M05Rik antibody
      • FLJ54750 antibody
      • Leftb antibody
      • OTTHUMP00000035614 antibody
      • P5C reductase 2 antibody
      • P5CR 2 antibody
      • P5CR2 antibody
      • P5CR2_HUMAN antibody
      • PYCR 2 antibody
      • PYCR2 antibody
      • Pyrroline 5 carboxylate reductase 2 antibody
      • Pyrroline 5 carboxylate reductase family member 2 antibody
      • Pyrroline 5 carboxylate reductase isoform antibody
      • Pyrroline-5-carboxylate reductase 2 antibody
      • RP4-559A3.4 antibody
      see all

    Images

    • Western blot - Anti-PYCR2 antibody (ab103535)
      Western blot - Anti-PYCR2 antibody (ab103535)This image is courtesy of an anonymous abreview.
      All lanes : Anti-PYCR2 antibody (ab103535) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate- 18µg
      Lane 2 : HeLa whole cell lysate- 36µg
      Lane 3 : RPE-1 whole cell lysate- 18µg
      Lane 4 : RPE-1 whole cell lysate- 36µg

      Secondary
      All lanes : LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 35 kDa (why is the actual band size different from the predicted?)


      Exposure time: 7 minutes


      Blocking: 10% Milk

      See Abreview

    • Western blot - Anti-PYCR2 antibody (ab103535)
      Western blot - Anti-PYCR2 antibody (ab103535)
      Anti-PYCR2 antibody (ab103535) at 1/500 dilution + HeLa cell lysate at 50 µg

      Predicted band size: 34 kDa

    • Western blot - Anti-PYCR2 antibody (ab103535)
      Western blot - Anti-PYCR2 antibody (ab103535)
      All lanes : Anti-PYCR2 antibody (ab103535) at 1/500 dilution

      Lane 1 : PYCR2 transfected 293T cell line lysate
      Lane 2 : Non-transfected 293T cell line lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 34 kDa

    References

    ab103535 has not yet been referenced specifically in any publications.

    Publishing research using ab103535? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    1 Review or Q&A

    10
    Votes: Highest First

    Western blot

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    38 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (HeLa, RPE-1)
    Specification
    HeLa, RPE-1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted May 01 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up