Anti-PYCR2 antibody (ab103535)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PYCR2 antibody
See all PYCR2 primary antibodies -
Description
Rabbit polyclonal to PYCR2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein, corresponding to amino acids 1-320 of Human PYCR2 (NP_037460.2).
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Positive control
- HeLa cell lysate; PYCR2 transfected 293T cell line lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab103535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 34 kDa. |
Target
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Function
Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. -
Tissue specificity
Detected in erythrocytes (at protein level). -
Pathway
Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1. -
Sequence similarities
Belongs to the pyrroline-5-carboxylate reductase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 29920 Human
- SwissProt: Q96C36 Human
- Unigene: 654718 Human
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Alternative names
- 1810018M05Rik antibody
- FLJ54750 antibody
- Leftb antibody
see all
Images
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Western blot - Anti-PYCR2 antibody (ab103535)This image is courtesy of an anonymous abreview.All lanes : Anti-PYCR2 antibody (ab103535) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate- 18µg
Lane 2 : HeLa whole cell lysate- 36µg
Lane 3 : RPE-1 whole cell lysate- 18µg
Lane 4 : RPE-1 whole cell lysate- 36µg
Secondary
All lanes : LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 7 minutesBlocking: 10% Milk
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Western blot - Anti-PYCR2 antibody (ab103535)Anti-PYCR2 antibody (ab103535) at 1/500 dilution + HeLa cell lysate at 50 µg
Predicted band size: 34 kDa -
Western blot - Anti-PYCR2 antibody (ab103535)All lanes : Anti-PYCR2 antibody (ab103535) at 1/500 dilution
Lane 1 : PYCR2 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa
Protocols
Datasheets and documents
References
ab103535 has not yet been referenced specifically in any publications.
