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    pycr2-antibody-ab103535.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-PYCR2 antibody (ab103535)

  • Datasheet
Reviews (3) Submit a question References (1)

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Western blot - Anti-PYCR2 antibody (ab103535)
  • Western blot - Anti-PYCR2 antibody (ab103535)
  • Western blot - Anti-PYCR2 antibody (ab103535)

Key features and details

  • Rabbit polyclonal to PYCR2
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human PYCR2 protein (ab162262)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PYCR2 antibody
    See all PYCR2 primary antibodies
  • Description

    Rabbit polyclonal to PYCR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-320 of Human PYCR2 (NP_037460.2).

  • Positive control

    • HeLa cell lysate; PYCR2 transfected 293T cell line lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PYCR2 protein (ab162262)

Applications

Our Abpromise guarantee covers the use of ab103535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.

Target

  • Function

    Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH.
  • Tissue specificity

    Detected in erythrocytes (at protein level).
  • Pathway

    Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
  • Sequence similarities

    Belongs to the pyrroline-5-carboxylate reductase family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q96C36 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29920 Human
    • SwissProt: Q96C36 Human
    • Unigene: 654718 Human
    • Alternative names

      • 1810018M05Rik antibody
      • FLJ54750 antibody
      • Leftb antibody
      • OTTHUMP00000035614 antibody
      • P5C reductase 2 antibody
      • P5CR 2 antibody
      • P5CR2 antibody
      • P5CR2_HUMAN antibody
      • PYCR 2 antibody
      • PYCR2 antibody
      • Pyrroline 5 carboxylate reductase 2 antibody
      • Pyrroline 5 carboxylate reductase family member 2 antibody
      • Pyrroline 5 carboxylate reductase isoform antibody
      • Pyrroline-5-carboxylate reductase 2 antibody
      • RP4-559A3.4 antibody
      see all

    Images

    • Western blot - Anti-PYCR2 antibody (ab103535)
      Western blot - Anti-PYCR2 antibody (ab103535)This image is courtesy of an anonymous abreview.
      All lanes : Anti-PYCR2 antibody (ab103535) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate- 18µg
      Lane 2 : HeLa whole cell lysate- 36µg
      Lane 3 : RPE-1 whole cell lysate- 18µg
      Lane 4 : RPE-1 whole cell lysate- 36µg

      Secondary
      All lanes : LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?


      Exposure time: 7 minutes


      Blocking: 10% Milk

      See Abreview

    • Western blot - Anti-PYCR2 antibody (ab103535)
      Western blot - Anti-PYCR2 antibody (ab103535)
      Anti-PYCR2 antibody (ab103535) at 1/500 dilution + HeLa cell lysate at 50 µg

      Predicted band size: 34 kDa

    • Western blot - Anti-PYCR2 antibody (ab103535)
      Western blot - Anti-PYCR2 antibody (ab103535)
      All lanes : Anti-PYCR2 antibody (ab103535) at 1/500 dilution

      Lane 1 : PYCR2 transfected 293T cell line lysate
      Lane 2 : Non-transfected 293T cell line lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 34 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab103535? Please let us know so that we can cite the reference in this datasheet.

    ab103535 has been referenced in 1 publication.

    • Keeratichamroen S  et al. Identification of potential cervical cancer serum biomarkers in Thai patients. Oncol Lett 19:3815-3826 (2020). PubMed: 32391095

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-PYCR2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Tissue lysate - other (Liver)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    60 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 21 2019

    Western blot abreview for Anti-PYCR2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 19 2019

    Western blot

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    38 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (HeLa, RPE-1)
    Specification
    HeLa, RPE-1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 01 2013

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