Anti-PYCR2 antibody (ab103535)
Key features and details
- Rabbit polyclonal to PYCR2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PYCR2 antibody
See all PYCR2 primary antibodies -
Description
Rabbit polyclonal to PYCR2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein, corresponding to amino acids 1-320 of Human PYCR2 (NP_037460.2).
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Positive control
- HeLa cell lysate; PYCR2 transfected 293T cell line lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab103535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 34 kDa. |
Target
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Function
Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. -
Tissue specificity
Detected in erythrocytes (at protein level). -
Pathway
Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1. -
Sequence similarities
Belongs to the pyrroline-5-carboxylate reductase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 29920 Human
- SwissProt: Q96C36 Human
- Unigene: 654718 Human
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Alternative names
- 1810018M05Rik antibody
- FLJ54750 antibody
- Leftb antibody
see all
Images
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Western blot - Anti-PYCR2 antibody (ab103535)This image is courtesy of an anonymous abreview.All lanes : Anti-PYCR2 antibody (ab103535) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate- 18µg
Lane 2 : HeLa whole cell lysate- 36µg
Lane 3 : RPE-1 whole cell lysate- 18µg
Lane 4 : RPE-1 whole cell lysate- 36µg
Secondary
All lanes : LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 7 minutesBlocking: 10% Milk
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Western blot - Anti-PYCR2 antibody (ab103535)Anti-PYCR2 antibody (ab103535) at 1/500 dilution + HeLa cell lysate at 50 µg
Predicted band size: 34 kDa -
Western blot - Anti-PYCR2 antibody (ab103535)All lanes : Anti-PYCR2 antibody (ab103535) at 1/500 dilution
Lane 1 : PYCR2 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa
Datasheets and documents
References (1)
ab103535 has been referenced in 1 publication.
- Keeratichamroen S et al. Identification of potential cervical cancer serum biomarkers in Thai patients. Oncol Lett 19:3815-3826 (2020). PubMed: 32391095
