Product nameAnti-PYGL antibody
See all PYGL primary antibodies
DescriptionRabbit polyclonal to PYGL
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Fusion protein corresponding to Human PYGL (C terminal). The identity of the protein fusion partner is GST (BC082229).
Database link: P06737
- 231 and PC3 cell lysates. Human thyroid cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab198268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/30 - 1/150.|
|WB||1/500 - 1/2000. Predicted molecular weight: 97 kDa.|
FunctionPhosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Involvement in diseaseDefects in PYGL are the cause of glycogen storage disease type 6 (GSD6) [MIM:232700]. A metabolic disorder characterized by mild to moderate hypoglycemia, mild ketosis, growth retardation, and prominent hepatomegaly. Heart and skeletal muscle are not affected.
Sequence similaritiesBelongs to the glycogen phosphorylase family.
modificationsPhosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
- Information by UniProt
- Glycogen phosphorylase antibody
- Glycogen phosphorylase L antibody
- Glycogen phosphorylase liver antibody
All lanes : Anti-PYGL antibody (ab198268) at 1/400 dilution
Lane 1 : 231 cell lysate
Lane 2 : PC3 cell lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 20 seconds
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PYGL with ab198268 at 1/35 dilution.
ab198268 has been referenced in 2 publications.
- Hughey CC et al. Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation. Mol Metab 23:1-13 (2019). PubMed: 30850319
- Liang X et al. Organic cation transporter 1 (OCT1) modulates multiple cardiometabolic traits through effects on hepatic thiamine content. PLoS Biol 16:e2002907 (2018). PubMed: 29659562