Key features and details
- Rabbit polyclonal to PYGL
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PYGL antibody
See all PYGL primary antibodies
DescriptionRabbit polyclonal to PYGL
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow
- WB: U-251 MG cell lysate. IHC-P: Human liver tissue. ICC/IF: U-251 MG cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 97 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPhosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Involvement in diseaseDefects in PYGL are the cause of glycogen storage disease type 6 (GSD6) [MIM:232700]. A metabolic disorder characterized by mild to moderate hypoglycemia, mild ketosis, growth retardation, and prominent hepatomegaly. Heart and skeletal muscle are not affected.
Sequence similaritiesBelongs to the glycogen phosphorylase family.
modificationsPhosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
- Information by UniProt
- Glycogen phosphorylase antibody
- Glycogen phosphorylase L antibody
- Glycogen phosphorylase liver antibody
Anti-PYGL antibody (ab223788) at 1/250 dilution + U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 97 kDa
Paraffin-embedded human liver tissue stained for PYGL using ab223788 at 1/500 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PYGL (green) using ab223788 at 4 µg/ml in ICC/IF.
ab223788 has been referenced in 1 publication.
- Liu X et al. Metformin and Berberine suppress glycogenolysis by inhibiting glycogen phosphorylase and stabilizing the molecular structure of glycogen in db/db mice. Carbohydr Polym 243:116435 (2020). PubMed: 32532388