Key features and details
- Rabbit polyclonal to PYGL - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PYGL antibody - C-terminal
See all PYGL primary antibodies
DescriptionRabbit polyclonal to PYGL - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
- HeLa, 293T and NIH 3T3 whole cell lysates
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab190243 was affinity purified using an epitope specific to PYGL immobilized on solid support.
Our Abpromise guarantee covers the use of ab190243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 97 kDa.|
|IP||Use at 1-10 µg/mg of lysate.|
FunctionPhosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Involvement in diseaseDefects in PYGL are the cause of glycogen storage disease type 6 (GSD6) [MIM:232700]. A metabolic disorder characterized by mild to moderate hypoglycemia, mild ketosis, growth retardation, and prominent hepatomegaly. Heart and skeletal muscle are not affected.
Sequence similaritiesBelongs to the glycogen phosphorylase family.
modificationsPhosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
- Information by UniProt
- Glycogen phosphorylase antibody
- Glycogen phosphorylase L antibody
- Glycogen phosphorylase liver antibody
All lanes : Anti-PYGL antibody - C-terminal (ab190243) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 3 minutes
Immunoprecipitation analysis of 293T cell lysate (in RIPA buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling PYGL using ab190243 at 6 μg per reaction (lane 1). a Control IgG was used in lane 2. For blotting immunoprecipitated PYGL, ab190243 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab190243 has been referenced in 1 publication.
- Ma J et al. Glycogen metabolism regulates macrophage-mediated acute inflammatory responses. Nat Commun 11:1769 (2020). PubMed: 32286295