Anti-PYGL antibody - C-terminal (ab190243)
Key features and details
- Rabbit polyclonal to PYGL - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PYGL antibody - C-terminal
See all PYGL primary antibodies -
Description
Rabbit polyclonal to PYGL - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide within Human PYGL aa 750 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P06737 -
Positive control
- HeLa, 293T and NIH 3T3 whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab190243 was affinity purified using an epitope specific to PYGL immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
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IP |
Use at 1-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 97 kDa. |
IP
Use at 1-10 µg/mg of lysate. |
Target
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Function
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. -
Involvement in disease
Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6) [MIM:232700]. A metabolic disorder characterized by mild to moderate hypoglycemia, mild ketosis, growth retardation, and prominent hepatomegaly. Heart and skeletal muscle are not affected. -
Sequence similarities
Belongs to the glycogen phosphorylase family. -
Post-translational
modificationsPhosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A. - Information by UniProt
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Database links
- Entrez Gene: 742309 Chimpanzee
- Entrez Gene: 5836 Human
- Entrez Gene: 110095 Mouse
- Entrez Gene: 706853 Rhesus monkey
- Omim: 613741 Human
- SwissProt: P06737 Human
- SwissProt: Q9ET01 Mouse
- Unigene: 282417 Human
see all -
Alternative names
- Glycogen phosphorylase antibody
- Glycogen phosphorylase L antibody
- Glycogen phosphorylase liver antibody
see all
Images
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All lanes : Anti-PYGL antibody - C-terminal (ab190243) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 3 minutes -
Immunoprecipitation analysis of 293T cell lysate (in RIPA buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling PYGL using ab190243 at 6 μg per reaction (lane 1). a Control IgG was used in lane 2. For blotting immunoprecipitated PYGL, ab190243 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab190243 has been referenced in 1 publication.
- Ma J et al. Glycogen metabolism regulates macrophage-mediated acute inflammatory responses. Nat Commun 11:1769 (2020). PubMed: 32286295