Key features and details
- Rabbit polyclonal to PYGM
- Suitable for: IHC-P, WB
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-PYGM antibody
See all PYGM primary antibodies
DescriptionRabbit polyclonal to PYGM
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Zebrafish
Recombinant fragment within Zebrafish PYGM aa 531-773. The exact sequence is proprietary.
Database link: 553655
- Zebrafish eye whole cell lysate; Zebrafish tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab210100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
|WB||1/500 - 1/3000. Predicted molecular weight: 97 kDa.|
FunctionPhosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Involvement in diseaseDefects in PYGM are the cause of glycogen storage disease type 5 (GSD5) [MIM:232600]; also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.
Sequence similaritiesBelongs to the glycogen phosphorylase family.
modificationsPhosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
- Information by UniProt
- Entrez Gene: 553655 Zebrafish
- Glycogen phosphorylase antibody
- Glycogen phosphorylase muscle form antibody
- muscle form antibody
ab210100 has not yet been referenced specifically in any publications.