Anti-PYGM antibody (ab231963)
Key features and details
- Rabbit polyclonal to PYGM
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PYGM antibody -
Description
Rabbit polyclonal to PYGM -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Cow, Cynomolgus monkey -
Immunogen
Recombinant fragment (His-tag) corresponding to Mouse PYGM aa 1-200. Expressed in E.coli.
Database link: Q9WUB3 -
Positive control
- WB: Recombinant mouse PYGM protein; mouse skeletal muscle and liver lysates; rat skeletal muscle lysate; HepG2 whole cell lysate (ab7900). IHC-P: Mouse skeletal muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 97 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 97 kDa. |
Target
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Function
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. -
Involvement in disease
Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) [MIM:232600]; also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria. -
Sequence similarities
Belongs to the glycogen phosphorylase family. -
Post-translational
modificationsPhosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A. - Information by UniProt
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Database links
- Entrez Gene: 5837 Human
- Entrez Gene: 19309 Mouse
- Entrez Gene: 24701 Rat
- Entrez Gene: 442998 Sheep
- Omim: 608455 Human
- SwissProt: P79334 Cow
- SwissProt: Q8HXW4 Cynomolgus monkey
- SwissProt: P11217 Human
see all -
Alternative names
- Glycogen phosphorylase antibody
- Glycogen phosphorylase muscle form antibody
- muscle form antibody
see all
Images
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Anti-PYGM antibody (ab231963) at 2 µg/ml + Recombinant mouse PYGM protein
Predicted band size: 97 kDaRecombinant fragment (His-tag) corresponding to Mouse PYGM aa 11-187, 22kDa.
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Anti-PYGM antibody (ab231963) at 2 µg/ml + Mouse skeletal muscle lysate
Predicted band size: 97 kDa -
Anti-PYGM antibody (ab231963) at 2 µg/ml + Rat skeletal muscle lysate
Predicted band size: 97 kDa -
Anti-PYGM antibody (ab231963) at 2 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 97 kDa -
Anti-PYGM antibody (ab231963) at 2 µg/ml + Mouse liver lysate
Predicted band size: 97 kDa -
Formalin-fixed, paraffin-embedded mouse skeletal muscle tissue stained for PYGM using ab231963 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab231963 has been referenced in 2 publications.
- Chen Z et al. Endometrial glycogen metabolism during early pregnancy in mice. Mol Reprod Dev 89:431-440 (2022). PubMed: 35842832
- Liu C et al. Glycogen Phosphorylase: A Drug Target of Amino Alcohols in Echinococcus granulosus, Predicted by a Computer-Aided Method. Front Microbiol 11:557039 (2020). PubMed: 33329421