Overview

  • Product name

  • Description

    Rabbit polyclonal to PYGM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH from the C terminal region of human PYGM.

  • Positive control

    • WB: 293 cell lysate. IHC-P: Human skeletal muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab63158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • Function

    Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
  • Involvement in disease

    Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) [MIM:232600]; also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.
  • Sequence similarities

    Belongs to the glycogen phosphorylase family.
  • Post-translational
    modifications

    Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glycogen phosphorylase antibody
    • Glycogen phosphorylase muscle form antibody
    • muscle form antibody
    • Muscpho antibody
    • Myophosphorylase antibody
    • Phosphorylase glycogen muscle (McArdle syndrome glycogen storage disease type V) antibody
    • Pygm antibody
    • PYGM_HUMAN antibody
    see all

Images

  • ab63158, at a 1/10 dilution, staining PYGM in formalin fixed, paraffin embedded human skeletal muscle tissue by Immunohistochemistry. ab63158 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
  • All lanes : Anti-PYGM antibody (ab63158) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysates
    Lane 2 : Lysates of 293 cells transiently transfected with PYGM gene.

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

References

This product has been referenced in:

  • García-Consuegra I  et al. Missense mutations have unexpected consequences: The McArdle disease paradigm. Hum Mutat 39:1338-1343 (2018). Read more (PubMed: 30011114) »
  • Nogales-Gadea G  et al. Expression of Glycogen Phosphorylase Isoforms in Cultured Muscle from Patients with McArdle's Disease Carrying the p.R771PfsX33 PYGM Mutation. PLoS One 5: (2010). WB ; Human . Read more (PubMed: 20957198) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry and your interest in our products.
The homology between mouse and human seems to be very high (above 95%) so cross-reactivity with murine PYGM can be expected even for those two antibodies (ab63158 and ab63159) which have not been tested in mouse.
The other antibody (ab88078) has been tested for cross-reaction with mouse but it has not been characterized for immunocyto/histochemistry. Since the host species is mouse, it may well be that non-specific high background could be a technical issue.

You may wish to take a look at this site for further information:
http://www.uniprot.org/blast/uniprot/201205082F6H7855XJ?alignment=15
We have launched an new scheme and can offer a testing discount for new species/application.
I would advise you to visit the following site where the Terms and Conditions of our testing initiative can be found at: www.abcam.com/collaborationdiscount.
I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

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