Overview

  • Product name

  • Description

    Rabbit polyclonal to PYGM
  • Host species

    Rabbit
  • Specificity

    Samples used to test ab81901 were: muscle and liver homogenates, purified glycogen. ab81901 will recogize glycogen phosphorylase from both liver and muscle. It has been used in ICC on human primary myotubes.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Dog
  • Immunogen

    KLH-conjugated peptide derived from the sequence of human glycogen phosphorylase.

  • Positive control

    • Rat liver homogenate. Human skeletal muscle homogenate. Muscle and liver homogenates, purified glycogen. Human primary myotubes

Applications

Our Abpromise guarantee covers the use of ab81901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 97 kDa.
IP Use at an assay dependent concentration.

Use at 5µg/500µg human skeletal muscle homogenate.ab81901 did not immunoprecipitate glycogen phosphorylase from rat liver.

Target

  • Function

    Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
  • Involvement in disease

    Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) [MIM:232600]; also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.
  • Sequence similarities

    Belongs to the glycogen phosphorylase family.
  • Post-translational
    modifications

    Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glycogen phosphorylase antibody
    • Glycogen phosphorylase muscle form antibody
    • muscle form antibody
    • Muscpho antibody
    • Myophosphorylase antibody
    • Phosphorylase glycogen muscle (McArdle syndrome glycogen storage disease type V) antibody
    • Pygm antibody
    • PYGM_HUMAN antibody
    see all

Images

  • All lanes : Anti-PYGM antibody (ab81901) at 1/1000 dilution

    Lane 1 : Rat liver homogenate at 50 µg
    Lane 2 : Purified rat liver glycogen at 10 µg
    Lane 3 : Human skeletal muscle homogenate at 50 µg
    Lane 4 : IP from 500 ug Human skeletal muscle homogenate

    Developed using the ECL technique.

    Predicted band size: 97 kDa


    Exposure time: 5 minutes

References

This product has been referenced in:

  • Feng YZ  et al. Loss of perilipin 2 in cultured myotubes enhances lipolysis and redirects the metabolic energy balance from glucose oxidation towards fatty acid oxidation. J Lipid Res 58:2147-2161 (2017). Read more (PubMed: 28822960) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your patience. The immunogen for ab81901 is from the human muscle isoform. It is 98% homologous with mouse muscle Glycogen Phosphorylase and 73% homologous with brain. I hope this helps, please let me know if you have any additional questions or concerns.

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Answer

Thank you for contacting us. Our antibodies to GPBB may preferentially recognize one form over another, for example ab116546 is 100% homologous with the brain isoform, but only 67% homologous with muscle. Because the sequences are very highly conserved, we do not currently have an antibody that would be guaranteed to be specific for only one form. I am sorry I could not be more helpful, please let me know if you need any additional information.

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