Overview

  • Product name

  • Description

    Rabbit polyclonal to PYGO2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human PYGO2 aa 1-368.
    Database link: Q9BRQ0

  • Positive control

    • IP: SW480 cell lysate. WB:NIH/3T3 cell lysate. IHC-P: Human lung adenocarcinoma tissue; mouse stomach tissue; rat intestine tissue. ICC/IF: HCT 116 cells.
  • General notes

     This product was previously labelled as Pygopus 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab155262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

Images

  • Anti-PYGO2 antibody (ab155262) at 1/1000 dilution + mouse NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 41 kDa



    10% SDS PAGE
  • Immunoprecipitation of PYGO2 from SW480 whole cell lysate (5 X 107 cells) using 1 ug of ab155262. The precipitated PYGO2 was detected using ab155262 diluted at 1:15000.
    Lane 1: IP-IgG
    Lane 2: IP-PYGO2 



    All lanes : Anti-PYGO2 antibody (ab155262) at 1/15000 dilution

    Lane 1 : IP negative control - SW480 whole cell lysate, corresponding to 5*107 cells, incubated with a IgG.
    Lane 2 : SW480 whole cell lysate, corresponding to 5*107 cells, immunoprecipitated with 1 µg of ab155262
  • 4% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells labeling PYGO2 using ab155262 at 1/500 dilution (green) in ICC/IF. 

    The nuclear counter stain is Hoechst 33342 (blue).

  • Paraffin-embedded rat intestine tissue labeleing PYGO2 using ab155262 at 1/500 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.

  • Paraffin-embedded mouse stomach tissue labeleing PYGO2 using ab155262 at 1/500 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung adenocarcinoma tissue, labeling PYGO2 using ab155262 at a 1/500 dilution.

References

ab155262 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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