Product nameAnti-PYGO2 antibody
See all PYGO2 primary antibodies
DescriptionRabbit polyclonal to PYGO2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human PYGO2 aa 1-50 (N terminal).
Database link: NP_612157.1
- HeLa, 293T and Mouse NIH3T3 whole cell lysates
This product was previously labelled as Pygopus 2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 41 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in signal transduction through the Wnt pathway.
Sequence similaritiesContains 1 PHD-type zinc finger.
- Information by UniProt
- 1190004M21Rik antibody
- FLJ33226 antibody
- PP7910 antibody
All lanes : Anti-PYGO2 antibody (ab99274) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PYGO2 with ab99274 at 1/200 (1µg/mg). Detection: DAB.
ab99274, at 0.4 µg/ml, detecting PYGO2 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
For immunoprecipitation, ab99274 was used at 3 µg/mg lysate (1 mg of lysate was used for IP and 20% of IP was loaded).
Lane 1; IP using ab99274
Lane 2; IP using a rabbit anti-PYGO2 antibody which recognizes a downstream epitope.
Lane 3; IP using control IgG.
ab99274 has not yet been referenced specifically in any publications.