Key features and details
- Rabbit polyclonal to PYGO2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PYGO2 antibody
See all PYGO2 primary antibodies
DescriptionRabbit polyclonal to PYGO2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human PYGO2 aa 1-50 (N terminal).
Database link: NP_612157.1
- HeLa, 293T and Mouse NIH3T3 whole cell lysates
This product was previously labelled as Pygopus 2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 41 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in signal transduction through the Wnt pathway.
Sequence similaritiesContains 1 PHD-type zinc finger.
- Information by UniProt
- 1190004M21Rik antibody
- FLJ33226 antibody
- PP7910 antibody
All lanes : Anti-PYGO2 antibody (ab99274) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PYGO2 with ab99274 at 1/200 (1µg/mg). Detection: DAB.
ab99274, at 0.4 µg/ml, detecting PYGO2 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
For immunoprecipitation, ab99274 was used at 3 µg/mg lysate (1 mg of lysate was used for IP and 20% of IP was loaded).
Lane 1; IP using ab99274
Lane 2; IP using a rabbit anti-PYGO2 antibody which recognizes a downstream epitope.
Lane 3; IP using control IgG.
ab99274 has not yet been referenced specifically in any publications.