Overview

  • Product name

  • Description

    Rabbit polyclonal to PYK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human PYK2 (internal sequence). The exact sequence is proprietary.
    Database link: Q14289

  • Positive control

    • WB: A431 and HeLa whole cell extracts; Mouse and rat brain extracts. IP: A431 whole cell extract. ICC/IF: HeLa cells. IHC-P: Rat brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab226798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 116 kDa.

Target

  • Function

    Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
  • Tissue specificity

    Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
  • Cellular localization

    Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
  • Information by UniProt
  • Database links

  • Alternative names

    • CADTK antibody
    • CAK-beta antibody
    • CAKB antibody
    • CAKbeta antibody
    • Calcium regulated non receptor proline rich tyrosine kinase antibody
    • Calcium-dependent tyrosine kinase antibody
    • Cell adhesion kinase beta antibody
    • E430023O05Rik antibody
    • EC 2.7.10.2 antibody
    • FADK 2 antibody
    • FADK2 antibody
    • FAK2 antibody
    • FAK2_HUMAN antibody
    • Focal adhesion kinase 2 antibody
    • MGC124628 antibody
    • PKB antibody
    • Proline-rich tyrosine kinase 2 antibody
    • Protein kinase B antibody
    • Protein Tyrosine Kinase 2 Beta antibody
    • Protein-tyrosine kinase 2-beta antibody
    • PTK antibody
    • PTK2B antibody
    • PTK2B protein tyrosine kinase 2 beta antibody
    • PYK2 antibody
    • RAFTK antibody
    • RAFTK2 antibody
    • Related adhesion focal tyrosine kinase antibody
    see all

Images

  • All lanes : Anti-PYK2 antibody (ab226798) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 116 kDa



    7.5% SDS-PAGE gel.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PYK2 (green) using ab226798 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded rat brain tissue stained for PYK2 using ab226798 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-PYK2 antibody (ab226798) at 1/1000 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Rat brain extract

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 116 kDa



    7.5% SDS-PAGE gel.

  • PYK2 was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab226798. Western blot was performed from the immunoprecipitate using ab226798.

    Lane 1: A431 whole cell extract.

    Lane 1: Control IgG IP in A431 whole cell extract.

    Lane 2: ab226798 IP in A431 whole cell extract.

    Anti-Rabbit IgG was used as a secondary reagent.

References

This product has been referenced in:

  • Sai C  et al. Cyclin Dependent Kinase 1 (CDK1) Activates Cardiac Fibroblasts via Directly Phosphorylating Paxillin at Ser244. Int Heart J 60:374-383 (2019). Read more (PubMed: 30745530) »
See 1 Publication for this product

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