• Product name

    Anti-PYK2 (phospho Y579 + Y580) antibody
    See all PYK2 primary antibodies
  • Description

    Rabbit polyclonal to PYK2 (phospho Y579 + Y580)
  • Host species

  • Specificity

    Some cross-reactivity still may be experienced in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 within the same cell system.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Chicken, Human
  • Immunogen

    Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosines 579 and 580. The sequence is conserved in human and rat.

  • Positive control

    • Vandate treated CEF cells expressing PYK 2 and plated on fibronectin.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: None
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2, (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence, and (iii) a phosphopeptide derived from the corresponding region of Focal Adhesion Kinase (a PYK 2-related protein) to remove antibody that is reactive with phosphorylated Focal Adhesion Kinase protein. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosines 579 and 580.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab4807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    WB: 1/1000. Predicted molecular weight: 116 kDa. Can be blocked with PYK2 peptide - phospho Y579 + Y580 (phospho and non-phospho pair) or CEF whole cell lysate, positive / negative for PYK 2 (pair).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
    • Tissue specificity

      Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • Post-translational

      Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
    • Cellular localization

      Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
    • Information by UniProt
    • Database links

    • Alternative names

      • CADTK antibody
      • CAK-beta antibody
      • CAKB antibody
      • CAKbeta antibody
      • Calcium regulated non receptor proline rich tyrosine kinase antibody
      • Calcium-dependent tyrosine kinase antibody
      • Cell adhesion kinase beta antibody
      • E430023O05Rik antibody
      • EC antibody
      • FADK 2 antibody
      • FADK2 antibody
      • FAK2 antibody
      • FAK2_HUMAN antibody
      • Focal adhesion kinase 2 antibody
      • MGC124628 antibody
      • PKB antibody
      • Proline-rich tyrosine kinase 2 antibody
      • Protein kinase B antibody
      • Protein Tyrosine Kinase 2 Beta antibody
      • Protein-tyrosine kinase 2-beta antibody
      • PTK antibody
      • PTK2B antibody
      • PTK2B protein tyrosine kinase 2 beta antibody
      • PYK2 antibody
      • RAFTK antibody
      • RAFTK2 antibody
      • Related adhesion focal tyrosine kinase antibody
      see all


    • Peptide Competition: Cell extracts prepared from chick embryo fibroblasts treated with vanadate were plated on fibronectin with (lanes 1-5) or without (lane 6) transfection of a PYK 2 expression vector and resolved by SDS PAGE on a 10% Tris-glycine gel. The proteins were then transferred to nitrocellulose. Membranes were incubated with 0.50 µg/mL ab4807, following prior incubation in the presence of the phosphopeptide immunogen (1), the absence of the phosphopeptide immunogen (2, 6), the non phosphopeptide corresponding to the PYK 2 phosphopeptide (3), the phosphopeptide corresponding to PYK 2 [pY579] (4), and the phosphopeptide corresponding to PYK 2 [pY580] (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the dual-phosphopeptide corresponding to this site blocks the antibody signal, not the corresponding mono phosphopeptides, t


    ab4807 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As


    On gel systems that resolve FAK (pp125) and Pyk2 (pp116), they will show up as a distinct doublet. If there is a lot of FAK relative to Pyk2, the normally low level of cross-reactivity (due to the fact that the antibodies are negatively adsorbed with the irrelevant phosphopeptides during our manufacturing process) can become a problem. For example, if FAK is present at levels 20-fold greater than Pyk2, then even if the Pyk2 antibodies are 95% selective for Pyk2 vs. FAK, you would see an equal signal for the two proteins.
    The reactivity for FAK is removed by absorption with a phosphopeptide with a corresponding sequence in FAK. This kind of adsorption will not be 100% that is why in the cells overexpress with FAK you will see signal.

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