Overview

  • Product name

  • Description

    Rabbit polyclonal to PYM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human PYM aa 94-158.
    Sequence:

    KEKRRQQQEKGEAEALSRTLDKVSLEETAQLPSAPQGSRAAPTAASDQPD SAATTEKAKKIKNLK


    Database link: Q9BRP8

  • Positive control

    • U-2 OS cells.
  • General notes

    Previously labelled as WIBG. 

Properties

Applications

Our Abpromise guarantee covers the use of ab220222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC.
  • Sequence similarities

    Belongs to the pym family.
  • Domain

    The eIF2A-like region shares sequence similarity with eIF2A and mediates the interaction with the 40S ribosomal subunit and the 48S preinitiation complex.
  • Cellular localization

    Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Shuttles between the nucleus and the cytoplasm (PubMed:14968132). Nuclear export is mediated by XPO1/CRM1 (PubMed:14968132).
  • Information by UniProt
  • Database links

  • Alternative names

    • A030010B05Rik antibody
    • MGC13064 antibody
    • Partner of Y14 and mago antibody
    • Protein wibg homolog antibody
    • PYM antibody
    • PYM homolog 1 exon junction complex-associated factor antibody
    • pym1 antibody
    • PYM1_HUMAN antibody
    • WIBG antibody
    • Within bgcn homolog antibody
    see all

Images

  • Immunofluorescence analysis of U-2 OS cells (PFA-fixed/Triton X-100 permeabilized) labeling PYM with ab220222 at 4 µg/mL (green).

References

ab220222 has not yet been referenced specifically in any publications.

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