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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Anti-PYM antibody (ab251913)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYM antibody (ab251913)
  • Western blot - Anti-PYM antibody (ab251913)

Key features and details

  • Rabbit polyclonal to PYM
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human PYM protein (ab172179)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PYM antibody
    See all PYM primary antibodies
  • Description

    Rabbit polyclonal to PYM
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human PYM aa 50-200.
    Database link: Q9BRP8

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human colon tissue. WB: HEK-293T overexpressing humnan PYN with a C-terminal myc-DDK tag, whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Recombinant Protein

    • Recombinant Human PYM protein (ab172179)
  • Related Products

    • Recombinant Human PYM protein (ab172179)
    • Recombinant Human PYM protein (ab98083)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 23 kDa.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 23 kDa.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC.
  • Sequence similarities

    Belongs to the pym family.
  • Domain

    The eIF2A-like region shares sequence similarity with eIF2A and mediates the interaction with the 40S ribosomal subunit and the 48S preinitiation complex.
  • Cellular localization

    Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Shuttles between the nucleus and the cytoplasm (PubMed:14968132). Nuclear export is mediated by XPO1/CRM1 (PubMed:14968132).
  • Target information above from: UniProt accession Q9BRP8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 84305 Human
    • SwissProt: Q9BRP8 Human
    • Unigene: 505687 Human
    • Unigene: 524488 Human
    • Alternative names

      • A030010B05Rik antibody
      • MGC13064 antibody
      • Partner of Y14 and mago antibody
      • Protein wibg homolog antibody
      • PYM antibody
      • PYM homolog 1 exon junction complex-associated factor antibody
      • pym1 antibody
      • PYM1_HUMAN antibody
      • WIBG antibody
      • Within bgcn homolog antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYM antibody (ab251913)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYM antibody (ab251913)
      Paraffin-embedded human colon tissue stained for PYM using ab251913 at 1/50 dilution in immunohistochemical analysis.
    • Western blot - Anti-PYM antibody (ab251913)
      Western blot - Anti-PYM antibody (ab251913)
      All lanes : Anti-PYM antibody (ab251913) at 0.4 µg/ml

      Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : HEK-293T overexpressing humnan PYN with a C-terminal myc-DDK tag, whole cell lysate

      Predicted band size: 23 kDa

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab251913? Please let us know so that we can cite the reference in this datasheet.

    ab251913 has not yet been referenced specifically in any publications.

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