Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Pyrin antibody [EPR18675] (ab214772)

Overview

  • Product name

    Anti-Pyrin antibody [EPR18675]
    See all Pyrin primary antibodies
  • Description

    Rabbit monoclonal [EPR18675] to Pyrin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment. within Mouse Pyrin aa 500-750. The exact sequence is proprietary.
    Database link: Q9JJ26

  • Positive control

    • WB: DC2.4 stable Pyrin expression whole cell lysate; Bone marrow derived-macrophage of wild type C57/B6 mice, untreated or stimulated with LPS. IP: DC2.4 stable Pyrin expression whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab214772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 86 kDa).
IP 1/30.

Target

  • Function

    Probably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization.
  • Tissue specificity

    Expressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes but not in lymphocytes. Detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues. Not expressed in thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, liver, kidney, pancreas. Expression detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines.
  • Involvement in disease

    Defects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF) [MIM:249100]. ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence.
    Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF) [MIM:134610]. ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness.
  • Sequence similarities

    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 DAPIN domain.
  • Developmental stage

    First detected in bone marrow promyelocytes. Expression increases throughout myelocyte differentiation and peaks in the mature myelomonocytic cells.
  • Cellular localization

    Nucleus and Cytoplasm > cytoskeleton. Associated with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles.
  • Information by UniProt
  • Database links

  • Alternative names

    • FMF antibody
    • Marenostrin antibody
    • Mediterranean fever antibody
    • Mediterranean fever protein antibody
    • MEF antibody
    • Mefv antibody
    • MEFV_HUMAN antibody
    • Pyrin antibody
    • TRIM20 antibody
    see all

Images

  • All lanes : Anti-Pyrin antibody [EPR18675] (ab214772) at 1/1000 dilution

    Lane 1 : DC2.4 (Mouse immature dendritic cell line) whole cell lysate
    Lane 2 : DC2.4 (Mouse immature dendritic cell line) stable mPyrin expression whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cells were kindly provided by our collaborator, Feng Shao, National institute of biological sciences, Beijing.

  • All lanes : Anti-Pyrin antibody [EPR18675] (ab214772) at 1/1000 dilution

    Lane 1 : Bone marrow derived-macrophage of Pyrin -/- mice
    Lane 2 : Bone marrow derived-macrophage of Pyrin -/- mice stimulated with LPS
    Lane 3 : Bone marrow derived-macrophage of wild type C57/B6 mice
    Lane 4 : Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with LPS

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The lysate is kindly provided by our collaborator, Feng Shao, National institute of biological sciences, Beijing.

  • Pyrin was immunoprecipitated from 0.35 mg of DC2.4 (Mouse immature dendritic cell line) stable mPyrin expression whole cell lysate with ab214772 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214772 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1:  ab214772 whole cell lysate from DC2.4 stable mPyrin expression cells 10µg (Input).

    Lane 2: ab214772 IP in whole cell lysate from DC2.4 stable mPyrin expression cells.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab214772 in whole cell lysate from DC2.4 stable mPyrin expression cells.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

References

ab214772 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (BMDM)
Total protein in input
5e+007 cells
Treatment
LPS
Immuno-precipitation step
Protein G
Specification
BMDM

Wenqing Gao

Verified customer

Submitted Jun 14 2017

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