Recombinant Anti-Pyrin antibody [EPR18676] - BSA and Azide free (ab222484)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18676] to Pyrin - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Pyrin antibody [EPR18676] - BSA and Azide free
See all Pyrin primary antibodies -
Description
Rabbit monoclonal [EPR18676] to Pyrin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Bone marrow derived-macrophage of wild type C57/B6 mice, untreated and stimulated with LPS or TNF alpha; DC2.4 stable expression whole cell lysate. IP: DC2.4 stable expression whole cell lysate.
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General notes
ab222484 is the carrier-free version of ab195975.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18676 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 86 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 86 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Probably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization. -
Tissue specificity
Expressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes but not in lymphocytes. Detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues. Not expressed in thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, liver, kidney, pancreas. Expression detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines. -
Involvement in disease
Defects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF) [MIM:249100]. ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence.
Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF) [MIM:134610]. ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness. -
Sequence similarities
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 DAPIN domain. -
Developmental stage
First detected in bone marrow promyelocytes. Expression increases throughout myelocyte differentiation and peaks in the mature myelomonocytic cells. -
Cellular localization
Nucleus and Cytoplasm > cytoskeleton. Associated with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles. - Information by UniProt
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Database links
- Entrez Gene: 54483 Mouse
- SwissProt: Q9JJ26 Mouse
- Unigene: 143718 Mouse
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Alternative names
- FMF antibody
- Marenostrin antibody
- Mediterranean fever antibody
see all
Images
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195975).
Pyrin was immunoprecipitated from DC2.4 (Mouse immature dendritic cell line) stable Pyrin expression whole cell lysate with ab195975 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab195975 at 1/1000 dilution. Goat anti-Rabbit IgG (H+L), was used as secondary antibody at 1/5000 dilution.
Lane 1: DC2.4 whole cell lysate.
Lane 2: DC2.4 stable Pyrin expression whole cell lysate.
Lane 3: DC2.4 stable Pyrin expression whole cell lysate.
Lane 4: ab195975 IP in DC2.4 whole cell lysate.
Lane 5: ab195975 IP in DC2.4 stable Pyrin expression whole cell lysate.
Lane 6: Mock IP (without ab195975) in DC2.4 stable Pyrin expression whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
The image was kindly provided by Dr Feng Shao's lab, NIBS.
All lanes : Anti-Pyrin antibody [EPR18676] (ab195975) at 1/100 dilution
Lane 1 : DC2.4 whole cell lysate.
Lanes 2-3 : DC2.4 stable Pyrin expression whole cell lysate.
Lane 4 : ab195975 IP in DC2.4 whole cell lysate.
Lane 5 : ab195975 IP in DC2.4 stable Pyrin expression whole cell lysate.
Lane 6 : Mock IP (without ab195975) in DC2.4 stable Pyrin expression whole cell lysate.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) at 1/5000 dilution
Exposure time: 30 seconds -
All lanes : Anti-Pyrin antibody [EPR18676] (ab195975) at 1/1000 dilution
Lane 1 : Bone marrow derived-macrophage of Pyrin -/- mice
Lane 2 : Bone marrow derived-macrophage of Pyrin -/- mice stimulated with LPS
Lane 3 : Bone marrow derived-macrophage of Pyrin -/- mice stimulated with TNF alpha
Lane 4 : Bone marrow derived-macrophage of wild type C57/B6 mice
Lane 5 : Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with LPS
Lane 6 : Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with TNF alpha
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) at 1/5000 dilution
Predicted band size: 86 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195975).
Blocking/Dilution buffer: 5% milk/TBST.
The image was kindly provided by Dr Feng Shao's lab, NIBS.
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab222484 has not yet been referenced specifically in any publications.