Recombinant
RabMAb

Recombinant Anti-Pyrin (phospho S241) antibody [EPR19570] (ab200420)

Overview

  • Product name

    Anti-Pyrin (phospho S241) antibody [EPR19570]
    See all Pyrin primary antibodies
  • Description

    Rabbit monoclonal [EPR19570] to Pyrin (phospho S241)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse Pyrin aa 200-300 (phospho S241). The exact sequence is proprietary.
    Database link: Q9JJ26

  • Positive control

    • WB: Cell lysate of DC2.4 expressing Pyrin.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab200420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 86 kDa).
Dot blot 1/1000.

Target

  • Function

    Probably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization.
  • Tissue specificity

    Expressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes but not in lymphocytes. Detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues. Not expressed in thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, liver, kidney, pancreas. Expression detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines.
  • Involvement in disease

    Defects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF) [MIM:249100]. ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence.
    Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF) [MIM:134610]. ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness.
  • Sequence similarities

    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 DAPIN domain.
  • Developmental stage

    First detected in bone marrow promyelocytes. Expression increases throughout myelocyte differentiation and peaks in the mature myelomonocytic cells.
  • Cellular localization

    Nucleus and Cytoplasm > cytoskeleton. Associated with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles.
  • Information by UniProt
  • Database links

  • Alternative names

    • FMF antibody
    • Marenostrin antibody
    • Mediterranean fever antibody
    • Mediterranean fever protein antibody
    • MEF antibody
    • Mefv antibody
    • MEFV_HUMAN antibody
    • Pyrin antibody
    • TRIM20 antibody
    see all

Images

  • Dot blot analysis of Pyrin (phospho S241) labeled with ab200420 at 1/1000 dilution.

    Lane 1: Pyrin (phospho S241) phospho peptide;

    Lane 2: Pyrin Non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • All lanes : Anti-Pyrin (phospho S241) antibody [EPR19570] (ab200420) at 1/1000 dilution

    Lane 1 : Cell lysate of DC2.4 (Mouse immature dendritic cell line) expressing mPyrin
    Lane 2 : Cell lysate of DC2.4 (Mouse immature dendritic cell line) expressing mPyrin. mPyrin dephosphorylation is stimulated with TcdA.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The lysates were kindly provided by our collaborator Feng Shao, National institute of biological sciences Beijing, China.

References

This product has been referenced in:

  • Gao W  et al. Site-specific phosphorylation and microtubule dynamics control Pyrin inflammasome activation. Proc Natl Acad Sci U S A 113:E4857-66 (2016). Read more (PubMed: 27482109) »
  • Van Gorp H  et al. Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation. Proc Natl Acad Sci U S A 113:14384-14389 (2016). Read more (PubMed: 27911804) »
See all 2 Publications for this product

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