Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19570] to Pyrin (phospho S241)
- Suitable for: WB, Dot blot
- Reacts with: Mouse
Product nameAnti-Pyrin (phospho S241) antibody [EPR19570]
See all Pyrin primary antibodies
DescriptionRabbit monoclonal [EPR19570] to Pyrin (phospho S241)
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Mouse
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse Pyrin aa 200-300 (phospho S241). The exact sequence is proprietary.
Database link: Q9JJ26
- WB: Cell lysate of DC2.4 expressing Pyrin.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab200420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 86 kDa).|
FunctionProbably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization.
Tissue specificityExpressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes but not in lymphocytes. Detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues. Not expressed in thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, liver, kidney, pancreas. Expression detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines.
Involvement in diseaseDefects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF) [MIM:249100]. ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence.
Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF) [MIM:134610]. ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness.
Sequence similaritiesContains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 DAPIN domain.
Developmental stageFirst detected in bone marrow promyelocytes. Expression increases throughout myelocyte differentiation and peaks in the mature myelomonocytic cells.
Cellular localizationNucleus and Cytoplasm > cytoskeleton. Associated with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles.
- Information by UniProt
- FMF antibody
- Marenostrin antibody
- Mediterranean fever antibody
Dot blot analysis of Pyrin (phospho S241) labeled with ab200420 at 1/1000 dilution.
Lane 1: Pyrin (phospho S241) phospho peptide;
Lane 2: Pyrin Non-phospho peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes : Anti-Pyrin (phospho S241) antibody [EPR19570] (ab200420) at 1/1000 dilution
Lane 1 : Cell lysate of DC2.4 (Mouse immature dendritic cell line) expressing mPyrin
Lane 2 : Cell lysate of DC2.4 (Mouse immature dendritic cell line) expressing mPyrin. mPyrin dephosphorylation is stimulated with TcdA.
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 1 second
Blocking/Dilution buffer: 5% NFDM/TBST.
The lysates were kindly provided by our collaborator Feng Shao, National institute of biological sciences Beijing, China.
ab200420 has been referenced in 3 publications.
- Magnotti F et al. Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients. EMBO Mol Med 11:e10547 (2019). PubMed: 31589380
- Gao W et al. Site-specific phosphorylation and microtubule dynamics control Pyrin inflammasome activation. Proc Natl Acad Sci U S A 113:E4857-66 (2016). PubMed: 27482109
- Van Gorp H et al. Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation. Proc Natl Acad Sci U S A 113:14384-14389 (2016). PubMed: 27911804