Recombinant Anti-Pyrin (phospho S241) antibody [EPR19570] (ab200420)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19570] to Pyrin (phospho S241)
- Suitable for: WB, Dot blot
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Pyrin (phospho S241) antibody [EPR19570]
See all Pyrin primary antibodies -
Description
Rabbit monoclonal [EPR19570] to Pyrin (phospho S241) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Cell lysate of DC2.4 expressing Pyrin.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19570 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 86 kDa).
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Dot blot |
1/1000.
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Notes |
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WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 86 kDa). |
Dot blot
1/1000. |
Target
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Function
Probably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization. -
Tissue specificity
Expressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes but not in lymphocytes. Detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues. Not expressed in thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, liver, kidney, pancreas. Expression detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines. -
Involvement in disease
Defects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF) [MIM:249100]. ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence.
Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF) [MIM:134610]. ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness. -
Sequence similarities
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 DAPIN domain. -
Developmental stage
First detected in bone marrow promyelocytes. Expression increases throughout myelocyte differentiation and peaks in the mature myelomonocytic cells. -
Cellular localization
Nucleus and Cytoplasm > cytoskeleton. Associated with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles. - Information by UniProt
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Database links
- Entrez Gene: 54483 Mouse
- SwissProt: Q9JJ26 Mouse
- Unigene: 143718 Mouse
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Alternative names
- FMF antibody
- Marenostrin antibody
- Mediterranean fever antibody
see all
Images
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Dot blot analysis of Pyrin (phospho S241) labeled with ab200420 at 1/1000 dilution.
Lane 1: Pyrin (phospho S241) phospho peptide;
Lane 2: Pyrin Non-phospho peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (6)
ab200420 has been referenced in 6 publications.
- Mangan MSJ et al. Transcriptional licensing is required for Pyrin inflammasome activation in human macrophages and bypassed by mutations causing familial Mediterranean fever. PLoS Biol 20:e3001351 (2022). PubMed: 36342970
- Park YH et al. Ancient familial Mediterranean fever mutations in human pyrin and resistance to Yersinia pestis. Nat Immunol 21:857-867 (2020). PubMed: 32601469
- Sharma D et al. RIPK3 Promotes Mefv Expression and Pyrin Inflammasome Activation via Modulation of mTOR Signaling. J Immunol 205:2778-2785 (2020). PubMed: 32989095
- Magnotti F et al. Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients. EMBO Mol Med 11:e10547 (2019). PubMed: 31589380
- Gao W et al. Site-specific phosphorylation and microtubule dynamics control Pyrin inflammasome activation. Proc Natl Acad Sci U S A 113:E4857-66 (2016). PubMed: 27482109
- Van Gorp H et al. Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation. Proc Natl Acad Sci U S A 113:14384-14389 (2016). PubMed: 27911804