Recombinant Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Pyrophosphatase 1 antibody [EPR11281]
See all Pyrophosphatase 1 primary antibodies -
Description
Rabbit monoclonal [EPR11281] to Pyrophosphatase 1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, Flow Cyt, WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Pyrophosphatase 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15181 -
Positive control
- HeLa, HepG2, A431 and 293T cell lysates; 293T cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11281 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| WB | 1/1000 - 1/5000. Predicted molecular weight: 33 kDa. |
Target
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Tissue specificity
Expressed ubiquitously. -
Sequence similarities
Belongs to the PPase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5464 Human
- Entrez Gene: 67895 Mouse
- Entrez Gene: 294504 Rat
- Omim: 179030 Human
- SwissProt: Q15181 Human
- SwissProt: Q9D819 Mouse
- Unigene: 437403 Human
- Unigene: 28897 Mouse
see all -
Alternative names
- cytosolic inorganic pyrophosphatase antibody
- diphosphate phosphohydrolase antibody
- inorganic diphosphatase antibody
see all
Images
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![Western blot - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)](https://www.abcam.com/ps/products/175/ab175223/Images/ab175223-201602-anti-pyrophosphatase-1-antibody-epr11281-western-blot.jpg)
Western blot - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)All lanes : Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa -
![Immunoprecipitation - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)](https://www.abcam.com/ps/products/175/ab175223/Images/ab175223-201607-anti-pyrophosphatase-1-antibody-epr11281-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using ab175223.
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![Flow Cytometry - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)](https://www.abcam.com/ps/products/175/ab175223/Images/ab175223-201605-anti-pyrophosphatase-1-antibody-epr11281-flow-cytometry.jpg)
Flow Cytometry - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)Flow cytometric analysis of permeabilized 293T cells labeling Pyrophosphatase 1 with ab175223 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Datasheets and documents
References
ab175223 has not yet been referenced specifically in any publications.
