Recombinant Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11281] to Pyrophosphatase 1
- Suitable for: IP, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Pyrophosphatase 1 antibody [EPR11281]
See all Pyrophosphatase 1 primary antibodies -
Description
Rabbit monoclonal [EPR11281] to Pyrophosphatase 1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, Flow Cyt, WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Pyrophosphatase 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15181 -
Positive control
- HeLa, HepG2, A431 and 293T cell lysates; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11281 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP | 1/10 - 1/100. | |
Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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WB | 1/1000 - 1/5000. Predicted molecular weight: 33 kDa. |
Target
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Tissue specificity
Expressed ubiquitously. -
Sequence similarities
Belongs to the PPase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5464 Human
- Entrez Gene: 67895 Mouse
- Entrez Gene: 294504 Rat
- Omim: 179030 Human
- SwissProt: Q15181 Human
- SwissProt: Q9D819 Mouse
- Unigene: 437403 Human
- Unigene: 28897 Mouse
see all -
Alternative names
- cytosolic inorganic pyrophosphatase antibody
- diphosphate phosphohydrolase antibody
- inorganic diphosphatase antibody
see all
Images
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All lanes : Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa -
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using ab175223.
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Flow cytometric analysis of permeabilized 293T cells labeling Pyrophosphatase 1 with ab175223 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Protocols
Datasheets and documents
References (0)
ab175223 has not yet been referenced specifically in any publications.