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RecombinantRabMAb

Recombinant Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)

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Western blot - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)
  • Immunoprecipitation - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)
  • Flow Cytometry - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)
  • Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11281] to Pyrophosphatase 1
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
Product image
Recombinant Human Pyrophosphatase 1 protein (ab123186)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Pyrophosphatase 1 antibody [EPR11281]
    See all Pyrophosphatase 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11281] to Pyrophosphatase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, Flow Cyt, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Pyrophosphatase 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15181

  • Positive control

    • HeLa, HepG2, A431 and 293T cell lysates; 293T cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11281
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals

Associated products

  • Alternative Versions

    • Anti-Pyrophosphatase 1 antibody [EPR11281] - BSA and Azide free (ab249874)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HepG2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • A431 whole cell lysate (ab30132)
    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Pyrophosphatase 1 protein (ab123186)
  • Related Products

    • Recombinant Human Pyrophosphatase 1 protein (ab123186)

Applications

Our Abpromise guarantee covers the use of ab175223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Tissue specificity

      Expressed ubiquitously.
    • Sequence similarities

      Belongs to the PPase family.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q15181 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5464 Human
      • Entrez Gene: 67895 Mouse
      • Entrez Gene: 294504 Rat
      • Omim: 179030 Human
      • SwissProt: Q15181 Human
      • SwissProt: Q9D819 Mouse
      • Unigene: 437403 Human
      • Unigene: 28897 Mouse
      • Unigene: 106916 Rat
      see all
    • Alternative names

      • cytosolic inorganic pyrophosphatase antibody
      • diphosphate phosphohydrolase antibody
      • inorganic diphosphatase antibody
      • inorganic pyrophosphatase 1 antibody
      • Inorganic pyrophosphatase antibody
      • IOPPP antibody
      • IPYR_HUMAN antibody
      • PP antibody
      • PP1 antibody
      • PPA1 antibody
      • PPase antibody
      • Pyp antibody
      • pyrophosphatase, inorganic, 1 antibody
      • Pyrophosphate phospho hydrolase antibody
      • Pyrophosphate phospho-hydrolase antibody
      • SID6-8061 antibody
      see all

    Images

    • Western blot - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)
      Western blot - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)
      All lanes : Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223) at 1/1000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 33 kDa

    • Immunoprecipitation - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)
      Immunoprecipitation - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)

      Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using ab175223.

    • Flow Cytometry - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)
      Flow Cytometry - Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)

      Flow cytometric analysis of permeabilized 293T cells labeling Pyrophosphatase 1 with ab175223 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    • Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)
      Anti-Pyrophosphatase 1 antibody [EPR11281] (ab175223)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab175223? Please let us know so that we can cite the reference in this datasheet.

    ab175223 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab175223.
    Please use the links above to contact us or submit feedback about this product.

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