Recombinant
RabMAb

Recombinant Anti-Pyrophosphatase 1 antibody [EPR11282] (ab181126)

Overview

  • Product name

    Anti-Pyrophosphatase 1 antibody [EPR11282]
    See all Pyrophosphatase 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11282] to Pyrophosphatase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pyrophosphatase 1 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15181

  • Positive control

    • HeLa, HepG2, 293T or Molt4 lysate, HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 33 kDa.
Flow Cyt 1/290.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/60.

Target

  • Tissue specificity

    Expressed ubiquitously.
  • Sequence similarities

    Belongs to the PPase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • cytosolic inorganic pyrophosphatase antibody
    • diphosphate phosphohydrolase antibody
    • inorganic diphosphatase antibody
    • inorganic pyrophosphatase 1 antibody
    • Inorganic pyrophosphatase antibody
    • IOPPP antibody
    • IPYR_HUMAN antibody
    • PP antibody
    • PP1 antibody
    • PPA1 antibody
    • PPase antibody
    • Pyp antibody
    • pyrophosphatase, inorganic, 1 antibody
    • Pyrophosphate phospho hydrolase antibody
    • Pyrophosphate phospho-hydrolase antibody
    • SID6-8061 antibody
    see all

Images

  • All lanes : Anti-Pyrophosphatase 1 antibody [EPR11282] (ab181126) at 1/10000 dilution

    All lanes : Anti-Pyrophosphatase 1 antibody [EPR11282] (ab181126)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  • Flow cytometry analysis of HepG2 cells using ab181126 at a 1/290 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

  • Detection of Human Pyrophosphatase 1 by Western Blot of Immunoprecipitate. MOLT4 whole cell lysate loaded, ab181126 used for blotting immunoprecipitated Pyrophosphatase 1 at a 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.

References

ab181126 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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