Overview

  • Product name

    Pyrophosphate Assay Kit (Colorimetric / Fluorometric)
    See all Pyrophosphate kits
  • Detection method

    Colorimetric/Fluorometric
  • Sample type

    Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Lysate
  • Assay type

    Quantitative
  • Product overview

    Pyrophosphate Assay Kit (Colorimetric / Fluorometric) (ab234040) provides a fast, convenient and ultrasensitive method for determination of free inorganic pyrophosphate (PPi) levels in biological material. PPi produced during biotic processes is detected through a series of reactions which utilize a proprietary enzyme mix and probe, generating a stable product that can be quantified by either colorimetric or fluorometric readout. Generated fluorescence (Ex/Em = 535/587 nm) or color (OD: 570 nm) intensities are directly proportional to the concentrations of pyrophosphate, enabling precise measurements. Monomeric inorganic phosphate (Pi) does not interfere with the assay. This kit delivers an easy and robust method suitable for use in a variety of biological samples and can be performed in a convenient microtiter-plate format. The kit provides sufficient reagents for 100 fluorometric or 50 colorimetric assays, respectively. This kit can detect as low as 1.8 μM PPi in plasma and serum samples.


    Inorganic pyrophosphate (diphosphate, pyrophosphoric acid or PPi) is a small molecule formed during, or required as a substrate for, a number of biochemical reactions. Enzymatic reactions that generate PPi include ATP hydrolysis, DNA and RNA polymerization, cyclic AMP formation, and enzymatic activation of fatty acids to form their CoA esters. Conversely, enzymes that utilize PPi may include cyclases, hydrolases and ligases. Measurement of inorganic pyrophosphate (PPi) in plasma, serum and other biologic fluids is important in studies of bone metabolism, renal stone disease, and certain types of arthritis.

  • Platform

    Microplate reader

Properties

Images

  • Cells were extracted directly in the Assay Buffer.

  • Spike and recovery in 20 µL of normal human plasma. Plasma samples were spiked with 0.06 nmol of PPi Standard and assayed according to kit protocol yielding 96% PPi recovery.

  • Quantification of pyrophosphate in 20 µL of de-proteinized undiluted human serum and pooled plasma.

Protocols

References

ab234040 has not yet been referenced specifically in any publications.

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