Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [9H9AF5] (ab110330)


  • Product name
    Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [9H9AF5]
    See all Pyruvate Dehydrogenase E1-alpha subunit primary antibodies
  • Description
    Mouse monoclonal [9H9AF5] to Pyruvate Dehydrogenase E1-alpha subunit
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, In-Cell ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Recombinant full length Human Pyruvate Dehydrogenase E1-alpha subunit.

  • Positive control
    • Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; cultured, normal Human embryonic lung fibroblasts (strain MRC5); HL60 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab110330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF Use a concentration of 5 µg/ml. (heat-induced antigen-retrieval improves signal)
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

In-Cell ELISA Use a concentration of 8 µg/ml.


  • Function
    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Tissue specificity
  • Involvement in disease
    Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
    Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • ODPA_HUMAN antibody
    • PDH antibody
    • PDHA antibody
    • PDHA1 antibody
    • PDHCE1A antibody
    • PDHE1 A type I antibody
    • PDHE1-A type I antibody
    • PHE1A antibody
    • Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody
    • Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody
    • Pyruvate Dehydrogenase E1 alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody
    see all


  • Immunocytochemistry analysis using ab110330 at 5µg/ml staining Pyruvate Dehydrogenase E1-alpha subunit in cultured, normal Human embryonic lung fibroblasts (strain MRC5) followed by AlexaFluor® 488 goat anti-mouse IgG1 secondary antibody (2 µg/ml).
  • Immunocytochemical analysis of SH-SY5Y human neuroblastoma labeling Pyruvate dehydrogenase E1-alpha with ab110330 at 1/500 dilution.

    See Abreview

  • Flow cytometric analysis using ab110330 at 1µg/ml staining Pyruvate Dehydrogenase E1-alpha subunit in HL60 cells (blue). Isotype control antibody (red).
  • All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [9H9AF5] (ab110330) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 10 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 4 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : hepG2 cell lysate at 20 µg

    Predicted band size: 43 kDa


This product has been referenced in:
  • McLelland GL  et al. Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy. Elife 7:N/A (2018). Read more (PubMed: 29676259) »
  • Schatton D  et al. CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. J Cell Biol 216:675-693 (2017). WB ; Mouse . Read more (PubMed: 28188211) »

See all 32 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry,

I am sorry to confirm that this kit is no longer available, and apologise for the inconvenience.

I can confirm that we do sell the separate antibody components which you may like to consider:

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Western blot
Sheep Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Jennifer Bruce

Verified customer

Submitted Jun 12 2015

Western blot
Human Cell lysate - whole cell (Ovarian cancer cell lines)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Ovarian cancer cell lines
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 25 2015

Immuno-precipitation step
Protein A/G
Mouse Tissue lysate - whole (heart)
Total protein in input
1000 µg

Abcam user community

Verified customer

Submitted Feb 23 2015

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Human Cell (SH-SY5Y human neuroblastoma)
SH-SY5Y human neuroblastoma
Yes - 0.2% NP-40, 3min

Dr. George Allen

Verified customer

Submitted Dec 16 2013

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