Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (HeLa and U2OS: Purified mitochondria lysate from 9)
Loading amount
3e+006 cells
Specification
HeLa and U2OS: Purified mitochondria lysate from 9
Gel Running Conditions
Reduced Denaturing (8% polyacrylamide gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS-T

Secondary antibody

Name
Non-Abcam antibody was used: Dako goat anti rabbit HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL plus
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 45 kDa
Positive control
U2OS, HeLa, HEK293 purified mitochondrial lysates

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Submitted Aug 09 2012

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