Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody

Good
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Heart, left ventricle)
Loading amount
40 µg
Specification
Heart, left ventricle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/200
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 43 kDa

Additional data

Additional Notes
Lanes 1,6 and 11 were loaded with Bio-Rad MW Marker, the rest were loaded with human left ventricular lysates.

Dawn Bowles

Verified customer

Submitted Dec 27 2012

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