Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (Human lung adenocarcinoma epithelial cell line)
Specification
Human lung adenocarcinoma epithelial cell line
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-TWEEN/BSA
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate - 170-6
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 43 kDa
Exposure
2 minute(s) and 0 second(s)

Dr. Stamatia Pouliliou

Verified customer

Submitted Mar 23 2015

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