Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody

Excellent
Abreviews
Application
Western blot
Sample
Sheep Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% BSA
Dilution
1/500

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Detection

Detection method
ELC
Exposure
1 second(s)
Bands
Specific: 40 kDa

Additional data

Additional Notes
very strong would dilute primary 1:2000 next time
Abcam response
The immunogen sequence homology is high with Sheep sequence hence the chances of cross reactivity are high.

Jennifer Bruce

Verified customer

Submitted Jun 15 2015

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