Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular Smooth Muscle Cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Vascular Smooth Muscle Cell
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 21°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 43 kDa

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Submitted Jul 20 2016

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