Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, liver, retina, RPE)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Brain, liver, retina, RPE
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey Blocking Buffer
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye­ 800CW

Detection

Detection method
Licor Odyssey Clx
Negative control
Blank lane (not shown)
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 43 kDa
Positive control
HEK cells, mouse liver

Additional data

Additional Notes
Lane 1: HEK cells Lane 2: ARPE 19 cells Lane 3: Mouse brain Lane 4: Mouse liver Lane 5: Mouse retina Lane 6: Mouse RPE PVDF membrane RIPA lysis buffer with mixed phosphatase and protease inhibitors

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Submitted Jun 20 2017

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