Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Retina, RPE)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina, RPE
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey Blocking Buffer
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye­ 800CW

Detection

Detection method
Licor Odyssey Clx
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 43 kDa
Positive control
Retina

Additional data

Additional Notes
Note: The red bands are imaged on the same channel as the ladder and cannot be removed in order to supply an image with a ladder. They correspond to ab67042 for RPE65 Lane 1: Rat retina Lane 2: Rat RPE RIPA lysis buffer PVDF membrane

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Submitted Jul 25 2017

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