Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331)


  • Product name
    Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ]
    See all Pyruvate Dehydrogenase E1 beta subunit primary antibodies
  • Description
    Mouse monoclonal [17A5E2H8 ] to Pyruvate Dehydrogenase E1 beta subunit
  • Host species
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Porcine Pyruvate Dehydrogenase E1 beta subunit protein.

  • Positive control
    • Isolated mitochondria from Human, Bovine, Rat and Mouse hearts; HepG2 cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal duodenum.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab110331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.


  • Function
    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in disease
    Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp564K0164 antibody
    • mitochondrial antibody
    • ODPB_HUMAN antibody
    • pdhB antibody
    • PDHBD antibody
    • PDHE1 B antibody
    • PDHE1-B antibody
    • PHE1B antibody
    • Pyruvate dehydrogenase (lipoamide) beta antibody
    • Pyruvate dehydrogenase E1 beta polypeptide antibody
    • Pyruvate dehydrogenase E1 component subunit beta antibody
    • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
    see all


  • IHC image of Pyruvate Dehydrogenase E1 beta subunit staining in Human normal duodenum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110331, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 10 µg/ml
    Lane 2 : Isolated mitochondria from Bovine heart at 4 µg/ml
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg/ml
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg/ml
    Lane 5 : HepG2 cell lysate at 20 µg/ml

    Predicted band size: 39 kDa


This product has been referenced in:
  • Qi X  et al. Knockdown of prohibitin expression promotes glucose metabolism in eutopic endometrial stromal cells from women with endometriosis. Reprod Biomed Online 29:761-70 (2014). Read more (PubMed: 25444511) »
  • Halim ND  et al. Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons. Glia 58:1168-76 (2010). Read more (PubMed: 20544852) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - other (keratinocytes mitochondria fraction)
Loading amount
66 µg
keratinocytes mitochondria fraction
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Mar 26 2013

Thank you for your reply.
As long as your mutant construct contains the either amino acid regions 72-121 or 250-360, then you should be able to either ab84588 orab55574, as these antibodies will bind within these regions respectively.

Read More

Thank you for contacting Abcam.
Depending on the exact amino acids that are missing from your mutant construct, one of the Pyruvate Dehydrogenase E1 beta subunit antibodies we have should work for you. Below is the immunogen information for each of...

Read More

Thank you for calling Abcam.

I am sorry that you were having issues with ab110331 in western blot. As requested I have sent you ab55574 as a free of charge replacement, your new order number is ********.

Please let me know if there ...

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