Overview

  • Product name
    Anti-Pyruvate Dehydrogenase E1 beta subunit antibody
    See all Pyruvate Dehydrogenase E1 beta subunit primary antibodies
  • Description
    Rabbit polyclonal to Pyruvate Dehydrogenase E1 beta subunit
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-314 of Human Pyruvate Dehydrogenase E1 beta subunit (Uniprot ID: P11177).

  • Positive control
    • A549, H1299, HCT116 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab154541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in disease
    Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp564K0164 antibody
    • mitochondrial antibody
    • ODPB_HUMAN antibody
    • pdhB antibody
    • PDHBD antibody
    • PDHE1 B antibody
    • PDHE1-B antibody
    • PHE1B antibody
    • Pyruvate dehydrogenase (lipoamide) beta antibody
    • Pyruvate dehydrogenase E1 beta polypeptide antibody
    • Pyruvate dehydrogenase E1 component subunit beta antibody
    • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
    see all

Images

  • All lanes : Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab154541) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling Pyruvate Dehydrogenase E1 beta subunit with ab154541 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Pyruvate Dehydrogenase E1 beta subunit with ab154541 at 1/500 dilution.

References

ab154541 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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