Recombinant
RabMAb

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11097(B)] (ab155996)

Overview

  • Product name
    Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11097(B)]
    See all Pyruvate Dehydrogenase E1 beta subunit primary antibodies
  • Description
    Rabbit monoclonal [EPR11097(B)] to Pyruvate Dehydrogenase E1 beta subunit
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Pyruvate Dehydrogenase E1 beta subunit (Uniprot P11177).

  • Positive control
    • HepG2, HeLa, A375 and Human fetal heart lysates; Human colon tissue; Human thyroid gland carcinoma tissue; HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in disease
    Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp564K0164 antibody
    • mitochondrial antibody
    • ODPB_HUMAN antibody
    • pdhB antibody
    • PDHBD antibody
    • PDHE1 B antibody
    • PDHE1-B antibody
    • PHE1B antibody
    • Pyruvate dehydrogenase (lipoamide) beta antibody
    • Pyruvate dehydrogenase E1 beta polypeptide antibody
    • Pyruvate dehydrogenase E1 component subunit beta antibody
    • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
    see all

Images

  • ab155996 showing +ve staining in Human normal pancreas.
  • ab155996 showing +ve staining in Human ovarian carcinoma.
  • ab155996 showing +ve staining in Human normal kidney.
  • ab155996 showing +ve staining in Human ovarian carcinoma.
  • All lanes : Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11097(B)] (ab155996) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A375 cell lysate
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 35 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Pyruvate Dehydrogenase E1 beta subunit with ab155996 at 1/50 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Pyruvate Dehydrogenase E1 beta subunit with ab155996 at 1/50 dilution.
  • Immunofluorescence analysis of HeLa cells labeling Pyruvate Dehydrogenase E1 beta subunit with ab155996 at 1/100 dilution.

References

This product has been referenced in:
  • Jin X  et al. HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. Int J Mol Med 39:1409-1420 (2017). Read more (PubMed: 28487965) »
  • Mazar J  et al. MicroRNA 211 Functions as a Metabolic Switch in Human Melanoma Cells. Mol Cell Biol 36:1090-108 (2016). Read more (PubMed: 26787841) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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