Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902)


  • Product name
    Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit
    See all Pyruvate dehydrogenase kits
  • Detection method
  • Sample type
    Cell culture extracts, Tissue Lysate, Purified mitochondria
  • Assay type
    Enzyme activity
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902) can be used to determine the activity of PDH in a human, bovine, mouse, or rat sample. The PDH enzyme is immunocaptured within the wells of the microplate and activity is determined by following the reduction of NAD+ to NADH, coupled to the reduction of a reporter dye to yield a colored reaction product with an increase in absorbance at 450 nm at room temperature. Included for performance of the activity assay are buffer, detergent, reagent mix, and a 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

    This assay is optimized for use with whole tissue extract when the amount of total material available for assay is 20-100 µg or more. If using cell extract of cultured cells 1 mg of material is required due to the very low levels of enzyme and reduced levels of mitochondria in the extract.

    The PDH complex is relatively fragile and sensitive to detergent. Please follow the sample preparation steps provided in the protocol booklet. Other preparation methods may decrease PDH activity. Our Scientific Support team is happy to answer any questions about sample prep.

    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • Notes

    Store 20X Buffer, Detergent, and Microplate at 4°C.

    Store 5X Stabilizer at -20°C.

    Store 20X Reagent Mix, 100X Reagent Dye and 100X Coupler at -80°C.

  • Platform



  • Mitochondria, tissue extracts and whole cultured cell extracts all show linear relationships between signal and sample load at limiting concentrations. The rates shown were determined as change in OD over time, and these are best represented as change in milliOD per minute.
  • Example of microplate reader recorded data from bovine heart mitochondria (100 µg/well) (top trace) and 2-fold dilutions (stepwise lower traces) using Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902).
  • Schematic diagram showing the reaction involved in Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902).
  • Principle of Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902)



This product has been referenced in:
  • Scheffler K  et al. 8-oxoguanine DNA glycosylase (Ogg1) controls hepatic gluconeogenesis. DNA Repair (Amst) 61:56-62 (2018). Read more (PubMed: 29207315) »
  • Ravera S  et al. Concentration-dependent metabolic effects of metformin in healthy and Fanconi anemia lymphoblast cells. J Cell Physiol 233:1736-1751 (2018). Read more (PubMed: 28681917) »

See all 35 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. I have heard back from the lab: Yes, liver tissues were tested with ab109902; for example extract of mouse liver homogenate. For extract of liver homogenate, 100 – 500 ug/200 uL should be used, please see attached ex...

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The product was tested on mitochondria isolated from fetal sheep hearts. Activity was obtained with sheep heart mitochondria and the raw data ( starting from 12.5 ng/well to 400ng/well) is attached below.

Abcam user community

Verified customer

Submitted Jan 09 2018

It is not necessary to avoid EDTA in the protease inhibitor cocktail. EDTA should not affect the pyruvate dehydrogenase enzyme in the sample or the functioning of the assay.

Thank you for your reply.

The laboratory have advised that probably the best in this situation would be to maintain the DCA in all the cell washes after treatment and during the assay itself without NaF, so that full activation of PDH can be o...

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I contacted the laboratories who have confirmed that for phosphatase inhibition we recommend 10mM NaF for use with this kit. A 500mM stock solution can be prepared in water and stored at -20C.

ab65621 is an appropriate protease inhibitor...

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Please see below for further clarification regarding the following questions:

Is it OK to extract mitos from frozen tissues?

A: yes, you can extract mitos from frozen tissues but be aware that freezen/ thaw cycle would weaken the cell...

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Yes, you can use frozen tissues as sample for all three kits. As a reminder, please follow each individual protocol included with each kit to prepare samples for that particular assay.

Thank you for contacting us.

Nome of the reagents in this kit contain thymine.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Read More

Please find the answers to your questions from my colleagues at Miosciences, who are designing this kits:
1. The dephosphorylation modifications can be done after the immunocapture of the PDH enzyme to the plate. Many related concerns and question...

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Product ab110338 contains whole mitochondria in iso-osmotic buffer. There is no need to measure protein concentration of this product; the product is supplied at 5.5 mg/mL. Simply adjust the protein concentration with PBS to 5.3 mg/mL and add dete...

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1-10 of 78 Abreviews or Q&A


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