Anti-Pyruvate Kinase antibody (HRP) - Azide free (ab34554)


  • Product name

    Anti-Pyruvate Kinase antibody (HRP) - Azide free
  • Description

    Goat polyclonal to Pyruvate Kinase - Azide free (HRP)
  • Host species

  • Conjugation

  • Tested applications

    Suitable for: WB, ELISA, Electron Microscopy, Dot blotmore details
  • Species reactivity

    Reacts with: Rabbit
  • Immunogen

    Full length native protein (purified): Pyruvate Kinase from Rabbit muscle



Our Abpromise guarantee covers the use of ab34554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/15000 - 1/60000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 58 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Pyruvate kinase is also known as ATP:pyruvate phosphotransferase. There are 4 isoforms of piruvate kinase in mammals: L, R, M1 and M2. L type is the major isoform in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells. Defects in PKLR (pyruvate kinase L and R) are the cause of pyruvate kinase hyperactivity; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP. Also defects in PKLR are a cause of chronic nonspherocytic hemolytic anemia (CNSHA); also called hereditary nonspherocytic hemolytic anemia.
    • Cellular localization

    • Database links

    • Alternative names

      • Pyruvate kinase 3 antibody
      • CTHBP antibody
      • Cytosolic thyroid hormone-binding protein antibody
      • epididymis secretory protein Li 30 antibody
      • HEL-S-30 antibody
      • OIP3 antibody
      • OPA interacting protein 3 antibody
      • p58 antibody
      • PK, muscle type antibody
      • PK1 antibody
      • PK2 antibody
      • PKL antibody
      • PKLR antibody
      • PKM 2 antibody
      • PKM antibody
      • PKM2 antibody
      • PYKM antibody
      • Pyruvate kinase 1 antibody
      • Pyruvate kinase 2/3 antibody
      • Pyruvate kinase isozyme R/L antibody
      • pyruvate kinase isozymes L/R antibody
      • Pyruvate kinase isozymes M1/M2 antibody
      • pyruvate kinase isozymes R/L antibody
      • Pyruvate kinase liver and blood cell antibody
      • Pyruvate kinase liver and RBC antibody
      • Pyruvate kinase muscle antibody
      • Pyruvate kinase muscle isozyme antibody
      • pyruvate kinase PKM antibody
      • pyruvate kinase type L antibody
      • Pyruvate kinase, liver and red blood cell antibody
      • Pyruvate kinase, liver type antibody
      • Pyruvate kinase, muscle, 2 antibody
      • Pyruvate kinase, red cell type antibody
      • R type/L type pyruvate kinase antibody
      • Red cell/liver pyruvate kinase antibody
      • RPK antibody
      • TCB antibody
      • THBP1 antibody
      • Thyroid hormone binding protein cytosolic antibody
      • thyroid hormone-binding protein 1 antibody
      • tumor M2-PK antibody
      see all


    ab34554 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Western blot
    Loading amount
    100 cells
    Gel Running Conditions
    Reduced Denaturing (8%)
    Mouse Cell lysate - whole cell (Bone marrow-derived macrophages)
    Bone marrow-derived macrophages
    poly I:C 1 ug/ml for the indicated times (0, 6, 24 and 48 hours)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Feb 04 2015


    Thank you for contacting us.

    Please see below for the anwers to your questions:

    ab34554: This antibody has only been tested with rabbit samples so far. We do not know how well it might work with human samples, but the homology is quite high: 97%. In a case like this, you would be eligible to use our testing discount program. Please see for details below and let me know if you would like to receive a discount code.
    The antibody is HRP-conjugated, which means that the use of a secondary antibody is not necessary.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here:

    ab34574: Yes, you are correct. This is a mistake. Since this is a pan-PK antibody, the predicted MW is between 58-62 kDa depending on the isoform. I have updated the datasheet accordingly. This antibody would be guaranteed for human samples.

    We guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link:
    The recommended starting dilution for use in WB is 1/5000 - 1/10,000.

    For ab117943 andab123908, I will have to inquire with the lab about the concentration. Yes, both of them are backordered 1-2 weeks. This means, if you place an order for these products today you would receive them within 1-2 weeks.

    I hope this information is helpful to you so far. I will be in touch with more information as soon as I have it. Thank you for your patience.

    In the meantime, please do not hesitate to contact us if you need any more advice or information.

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