Product nameAnti-Pyruvate Kinase antibody (HRP) - Azide free
DescriptionGoat polyclonal to Pyruvate Kinase - Azide free (HRP)
Tested applicationsSuitable for: WB, ELISA, Electron Microscopy, Dot blotmore details
Species reactivityReacts with: Rabbit
Full length native protein (purified): Pyruvate Kinase from Rabbit muscle
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab34554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/15000 - 1/60000.
WB: Use at an assay dependent dilution. Predicted molecular weight: 58 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevancePyruvate kinase is also known as ATP:pyruvate phosphotransferase. There are 4 isoforms of piruvate kinase in mammals: L, R, M1 and M2. L type is the major isoform in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells. Defects in PKLR (pyruvate kinase L and R) are the cause of pyruvate kinase hyperactivity; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP. Also defects in PKLR are a cause of chronic nonspherocytic hemolytic anemia (CNSHA); also called hereditary nonspherocytic hemolytic anemia.
- Pyruvate kinase 3 antibody
- CTHBP antibody
- Cytosolic thyroid hormone-binding protein antibody
ab34554 has not yet been referenced specifically in any publications.