Key features and details
- Rabbit polyclonal to Pyruvate kinase isozyme M1
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Pyruvate kinase isozyme M1 antibody
See all Pyruvate kinase isozyme M1 primary antibodies
DescriptionRabbit polyclonal to Pyruvate kinase isozyme M1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Horse, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Mouse Pyruvate kinase isozyme M1 aa 350-450 conjugated to keyhole limpet haemocyanin.
Database link: P52480
- This antibody gave a positive signal in E14Tg2A whole cell lysate as well as the following Mouse tissue lysates: Skeletal Muscle; Heart; Brain. IHC-P: Mouse heart FFPE tissue sections.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab156849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).|
FunctionGlycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Tissue specificitySpecifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similaritiesBelongs to the pyruvate kinase family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Under hypoxia, hydroxylated by EGLN3.
Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
- Information by UniProt
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IHC image of Pyruvate kinase isozyme M1 staining in mouse heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab156849, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Pyruvate kinase isozyme M1 antibody (ab156849) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Additional bands at: 34 kDa (possible non-specific binding), 98 kDa (possible non-specific binding)
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156849 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab156849 has been referenced in 1 publication.
- Li Z et al. Establishment of a colorectal cancer nude mouse visualization model of HIF-1a overexpression. Oncol Lett 11:2725-2732 (2016). WB . PubMed: 27073543