Overview

  • Product name
    Anti-Pyruvate kinase isozyme M1 antibody
    See all Pyruvate kinase isozyme M1 primary antibodies
  • Description
    Rabbit polyclonal to Pyruvate kinase isozyme M1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Horse, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Mouse Pyruvate kinase isozyme M1 aa 350-450 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P52480

  • Positive control
    • This antibody gave a positive signal in E14Tg2A whole cell lysate as well as the following Mouse tissue lysates: Skeletal Muscle; Heart; Brain. IHC-P: Mouse heart FFPE tissue sections.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

Target

  • Function
    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • Tissue specificity
    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Sequence similarities
    Belongs to the pyruvate kinase family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
    Under hypoxia, hydroxylated by EGLN3.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTHBP antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • OIP-3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • pkm antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase isozymes M1/M2 antibody
    • Pyruvate kinase muscle isozyme antibody
    • THBP1 antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2-PK antibody
    see all

Images

  • IHC image of Pyruvate kinase isozyme M1 staining in mouse heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab156849, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Pyruvate kinase isozyme M1 antibody (ab156849) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa
    Additional bands at: 34 kDa (possible non-specific binding), 98 kDa (possible non-specific binding)


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156849 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:
  • Li Z  et al. Establishment of a colorectal cancer nude mouse visualization model of HIF-1a overexpression. Oncol Lett 11:2725-2732 (2016). WB . Read more (PubMed: 27073543) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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