Anti-QDPR/DHPR antibody (ab126150)
Key features and details
- Rabbit polyclonal to QDPR/DHPR
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-QDPR/DHPR antibody
See all QDPR/DHPR primary antibodies -
Description
Rabbit polyclonal to QDPR/DHPR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human QDPR/DHPR aa 1-221.
Database link: P09417 -
Positive control
- Jurkat whole cell lysate, Mouse liver whole cell lysate and Human colon cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
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IHC-P |
1/100 - 1/500. Perform antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer (pH8.0)
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
IHC-P
1/100 - 1/500. Perform antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer (pH8.0) |
Target
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Function
The product of this enzyme, tetrahydrobiopterin (BH-4), is an essential cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases. -
Involvement in disease
Defects in QDPR are the cause of BH4-deficient hyperphenylalaninemia type C (HPABH4C) [MIM:261630]; also called dihydropteridine reductase deficiency (DHPR deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to DHPR deficiency or quinoid dihydropteridine reductase deficiency (QDPR deficiency). HPABH4C is a rare autosomal recessive disorder characterized by hyperphenylalaninemia and severe neurologic symptoms (malignant hyperphenylalaninemia) including axial hypotonia and truncal hypertonia, abnormal thermogenesis, and microcephaly. These signs are attributable to depletion of the neurotransmitters dopamine and serotonin, whose syntheses are controlled by tryptophan and tyrosine hydroxylases that use BH-4 as cofactor. These patients do not respond to phenylalanine-restricted diet. HPABH4C is lethal if untreated. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. - Information by UniProt
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Database links
- Entrez Gene: 5860 Human
- Entrez Gene: 110391 Mouse
- Omim: 612676 Human
- SwissProt: P09417 Human
- SwissProt: Q8BVI4 Mouse
- Unigene: 75438 Human
- Unigene: 30204 Mouse
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Alternative names
- 6,7 dihydropteridine reductase antibody
- DHPR antibody
- DHPR_HUMAN antibody
see all
Images
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Anti-QDPR/DHPR antibody (ab126150) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Anti-QDPR/DHPR antibody (ab126150) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
ab126150, at 1/500 dilution, staining QDPR/DHPR in Formalin-fixed, Paraffin-embedded Human Colon cancer tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126150 has been referenced in 1 publication.
- Hung CY et al. A plant-based mutant huntingtin model-driven discovery of impaired expression of GTPCH and DHFR. Cell Mol Life Sci 79:553 (2022). PubMed: 36251090