Key features and details
- Rabbit polyclonal to QDPR/DHPR
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-QDPR/DHPR antibody
See all QDPR/DHPR primary antibodies
DescriptionRabbit polyclonal to QDPR/DHPR
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Rat, Human, Pig
Recombinant full length protein (His-tag) corresponding to Human QDPR/DHPR aa 1-244. (Expressed in E.coli).
MAAAAAAGEARRVLVYGGRGALGSRCVQAFRARNWWVASVDVVENEEASA SIIVKMTDSFTEQADQVTAEVGKLLGEEKVDAILCVAGGWAGGNAKSKSL FKNCDLMWKQSIWTSTISSHLATKHLKEGGLLTLAGAKAALDGTPGMIGY GMAKGAVHQLCQSLAGKNSGMPPGAAAIAVLPVTLDTPMNRKSMPEADFS SWTPLEFLVETFHDWITGKNRPSSGSLIQVVTTEGRTELTPAYF
Database link: P09417
- IHC-P: Human cerebrum tissue. ICC/IF: HepG2 cells. WB: Recombinant human QDPR/DHPR protein; HepG2 cell lysate; Pig liver and brain lysate; Rat liver lysate.
Previously labelled as QDPR.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/5 - 1/20.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 26 kDa.|
FunctionThe product of this enzyme, tetrahydrobiopterin (BH-4), is an essential cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases.
Involvement in diseaseDefects in QDPR are the cause of BH4-deficient hyperphenylalaninemia type C (HPABH4C) [MIM:261630]; also called dihydropteridine reductase deficiency (DHPR deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to DHPR deficiency or quinoid dihydropteridine reductase deficiency (QDPR deficiency). HPABH4C is a rare autosomal recessive disorder characterized by hyperphenylalaninemia and severe neurologic symptoms (malignant hyperphenylalaninemia) including axial hypotonia and truncal hypertonia, abnormal thermogenesis, and microcephaly. These signs are attributable to depletion of the neurotransmitters dopamine and serotonin, whose syntheses are controlled by tryptophan and tyrosine hydroxylases that use BH-4 as cofactor. These patients do not respond to phenylalanine-restricted diet. HPABH4C is lethal if untreated.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
- Information by UniProt
- 6,7 dihydropteridine reductase antibody
- DHPR antibody
- DHPR_HUMAN antibody
HepG2 (human liver hepatocellular carcinoma cell line) cells labelling QDPR/DHPR (green) using ab232983 at 20 ug/ml in ICC/IF. FITC staining.
All lanes : Anti-QDPR/DHPR antibody (ab232983) at 2 µg/ml
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate.
Lane 2 : Pig liver tissue lysate
Lane 3 : Pig brain tissue lysate.
Lane 4 : Rat brain tissue lysate.
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 26 kDa
Anti-QDPR/DHPR antibody (ab232983) at 2 µg/ml + Recombinant human QDPR/DHPR protein.
Predicted band size: 26 kDa
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for QDPR/DHPR using ab232983 at 20ug/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232983 has not yet been referenced specifically in any publications.