Recombinant
RabMAb

Recombinant Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)

Overview

  • Product name

    Anti-QKI antibody [EPR7306] - BSA and Azide free
    See all QKI primary antibodies
  • Description

    Rabbit monoclonal [EPR7306] to QKI - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human QKI aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96PU8

  • Positive control

    • ICC/IF: Neuro-2a cells.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab232502 is a PBS-only buffer format of ab126742. Please refer to ab126742 for recommended dilutions, protocols, and image data

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor.
  • Tissue specificity

    Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.
  • Sequence similarities

    Contains 1 KH domain.
  • Domain

    The KH domain and the Qua2 region are involved in RNA binding.
  • Post-translational
    modifications

    Methylated by PRMT1.
    Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp586I0923 antibody
    • HKQ antibody
    • Homolog of mouse quaking QKI KH domain RNA binding protein antibody
    • Hqk antibody
    • HQK1 antibody
    • HqkI antibody
    • OTTHUMP00000017581 antibody
    • OTTHUMP00000017582 antibody
    • OTTHUMP00000017583 antibody
    • Protein quaking antibody
    • QK antibody
    • QK1 antibody
    • QK3 antibody
    • QKI antibody
    • QKI_HUMAN antibody
    • QKI1 antibody
    • Quaking homolog antibody
    • Quaking homolog KH domain RNA binding antibody
    • Quaking homolog KH domain RNA binding mouse antibody
    • Quaking isoform 1 antibody
    • Quaking protein antibody
    • RNA binding protein HQK antibody
    see all

Images

  • ab126742 (purified) at 1:30 dilution (2µg) immunoprecipitating QKI in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab126742 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126742 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126742).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126742).

  • Immunofluorescent analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling QKI with Purified ab126742 at 1:100 dilution (5.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126742).

References

ab232502 has not yet been referenced specifically in any publications.

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