Anti-QKI antibody [N147/6] (ab186245)
Key features and details
- Mouse monoclonal [N147/6] to QKI
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-QKI antibody [N147/6]
See all QKI primary antibodies -
Description
Mouse monoclonal [N147/6] to QKI -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human QKI aa 1 to the C-terminus.
Database link: Q96PU8 -
Positive control
- WB: Rat brain membrane lysate; HAP1 whole cell lysate; HeLa whole cell lysate; THP-1 whole cell lysate. ICC/IF: SK-N-BE cells.
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General notes
The clone number has been updated from S147-6 to N147/6, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N147/6 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor. -
Tissue specificity
Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients. -
Sequence similarities
Contains 1 KH domain. -
Domain
The KH domain and the Qua2 region are involved in RNA binding. -
Post-translational
modificationsMethylated by PRMT1.
Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9444 Human
- Entrez Gene: 19317 Mouse
- Entrez Gene: 499022 Rat
- Omim: 609590 Human
- SwissProt: Q96PU8 Human
- SwissProt: Q9QYS9 Mouse
- SwissProt: Q91XU1 Rat
- Unigene: 510324 Human
see all -
Alternative names
- DKFZp586I0923 antibody
- HKQ antibody
- Homolog of mouse quaking QKI KH domain RNA binding protein antibody
see all
Images
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All lanes : Anti-QKI antibody [N147/6] (ab186245) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : QKI knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : THP-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 37 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab186245 observed at 39 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab186245 was shown to specifically react with in wild-type HAP1 cells as signal was lost in QKI knockout cells. Wild-type and QKI knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab186245 and ab52866 (Rabbit anti-tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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SK-N-BE (Human neuroblastoma cell line) cells stained for QKI using ab186245 at a dilution of 1/100 in ICC/IF.
Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary used is a Goat Anti-Mouse ATTO 488 at 1:100 dilution for 60 minutes at RT.
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Anti-QKI antibody [N147/6] (ab186245) at 1/200 dilution + Rat brain membrane lysate at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 6 minutesBlock: 2% BSA and 2% Skimmed milk in 1X TBST.
Primary antibody incubated for 16 hours at +4°C.
Secondary antibody incubated for 1 hour at RT.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab186245 has been referenced in 1 publication.
- Zhang B et al. A regulatory circuit of circ-MTO1/miR-17/QKI-5 inhibits the proliferation of lung adenocarcinoma. Cancer Biol Ther 20:1127-1135 (2019). PubMed: 30975029