Product nameAnti-QKI antibody [N147/6]
See all QKI primary antibodies
DescriptionMouse monoclonal [N147/6] to QKI
Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein corresponding to Human QKI aa 1-341.
MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEIS RVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVG RILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNED LHVLITVEDAQNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAIL NGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPT IMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSI LEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN
Database link: Q96PU8
- WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
The clone number has been updated from S147-6 to N147/6, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab186245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 36-38 kDa (predicted molecular weight: 38 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionRNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor.
Tissue specificityExpressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.
Sequence similaritiesContains 1 KH domain.
DomainThe KH domain and the Qua2 region are involved in RNA binding.
modificationsMethylated by PRMT1.
Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- DKFZp586I0923 antibody
- HKQ antibody
- Homolog of mouse quaking QKI KH domain RNA binding protein antibody
SK-N-BE (Human neuroblastoma cell line) cells stained for QKI using ab186245 at a dilution of 1/100 in ICC/IF.
Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary used is a Goat Anti-Mouse ATTO 488 at 1:100 dilution for 60 minutes at RT.
Anti-QKI antibody [N147/6] (ab186245) at 1/200 dilution + Rat brain membrane lysate at 15 µg
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 6 minutes
Block: 2% BSA and 2% Skimmed milk in 1X TBST.
Primary antibody incubated for 16 hours at +4°C.
Secondary antibody incubated for 1 hour at RT.
ab186245 has not yet been referenced specifically in any publications.