Key features and details
- Mouse monoclonal [N147/6] to QKI
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Product nameAnti-QKI antibody [N147/6]
See all QKI primary antibodies
DescriptionMouse monoclonal [N147/6] to QKI
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein corresponding to Human QKI aa 1-341.
MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEIS RVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVG RILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNED LHVLITVEDAQNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAIL NGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPT IMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSI LEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN
Database link: Q96PU8
- WB: Rat brain membrane lysate; HAP1 whole cell lysate; HeLa whole cell lysate; THP-1 whole cell lysate. ICC/IF: SK-N-BE cells.
The clone number has been updated from S147-6 to N147/6, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab186245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
FunctionRNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor.
Tissue specificityExpressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.
Sequence similaritiesContains 1 KH domain.
DomainThe KH domain and the Qua2 region are involved in RNA binding.
modificationsMethylated by PRMT1.
Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- DKFZp586I0923 antibody
- HKQ antibody
- Homolog of mouse quaking QKI KH domain RNA binding protein antibody
All lanes : Anti-QKI antibody [N147/6] (ab186245) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : QKI knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : THP-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 37 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Lanes 1 - 4: Merged signal (red and green). Green - ab186245 observed at 39 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab186245 was shown to specifically react with in wild-type HAP1 cells as signal was lost in QKI knockout cells. Wild-type and QKI knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab186245 and ab52866 (Rabbit anti-tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
SK-N-BE (Human neuroblastoma cell line) cells stained for QKI using ab186245 at a dilution of 1/100 in ICC/IF.
Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary used is a Goat Anti-Mouse ATTO 488 at 1:100 dilution for 60 minutes at RT.
Anti-QKI antibody [N147/6] (ab186245) at 1/200 dilution + Rat brain membrane lysate at 15 µg
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 6 minutes
Block: 2% BSA and 2% Skimmed milk in 1X TBST.
Primary antibody incubated for 16 hours at +4°C.
Secondary antibody incubated for 1 hour at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab186245 has not yet been referenced specifically in any publications.